Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...
Patent
1995-10-18
1997-03-25
Eisenschenk, Frank C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Animal cell, per se, expressing immunoglobulin, antibody, or...
436518, 5303885, 5303891, 435 71, C07K 1616, C12N 512, G01N 3353
Patent
active
056144083
ABSTRACT:
A hybridoma cell lines is described which produces and secretes a monoclonal antibody which selectively binds to the glycoalkaloids of potatoes, tomatoes, and eggplants, as well as their corresponding aglycones. Glycoalkaloids of potatoes, tomatoes, and/or eggplants in biological samples may be detected and quantified by contacting the sample with the antibodies to form a glycoalkaloid/antibody immunocomplex when the glycoalkaloids are present, which immunocomplex may then be detected. The monoclonal antibody may also be incorporated into kits for the detection and quantification of glycoalkaloids in plants, foods, and body tissues and fluids.
REFERENCES:
Stanker, Larry H., Kamps-Holtzapple, Carol, Freidman, Mendel, "Development and Characterization of Monoclonal Antibodies That Differentiate between Potato and Tomato Gylcoalkaloids and Aglycons." Journal of Agricultural and Food Chemistry Oct. 19, 1994, vol. 42, pp. 2360-2366, American Chemical Society.
Thomson, Carrie A., Sporns, Peter, "Fluorescence Polarization Immunoassays for potato Glycoalkaloids," Journal of Agricultural and Food Chemistry, 1995, vol. 43, pp. 254-260, American Chemical Society.
Pihak, Leslie C., Sporns, Peter, "Enzyme Immunoassay for Potato Glycoalkaloids", Journal of Agricultural and Food Chemistry, 1992, vol. 40, pp. 2533-2540, American Chemical Society.
Phlak, Leslie, and Sporns, Peter, "Development and Production of Monoclonal Antibodies for the Measurement of Solanidine Potato Glycoalkaloids", American Potato Journal, 1994, vol. 71, No. 5, pp. 297-313.
Stanker, Larry H., Kampa-Holtzapple, C, Friedman, M., Abstract entitled "Monoclonal Antibodies to Potato Glycoalkaloids and Molecular Modeling Studies of Cross-reacting Compounds", Presented at the annual meeting of the American Chemical Society in Mar. 1993.
Friedman, Mendel, and Levin, Carol E., ".alpha.-Tomatine Content in Tomato and Tomato Products Determined by HPLC with Pulsed Amperometric Detection", Journal of Agricultural and Food Chemistry, 1995, vol. 43, No. 6, pp. 1507-1511.
Friedman Mendel
Holtzapple Carol K.
Stanker Larry H.
Deck Randall E.
Eisenschenk Frank C.
Fado John D.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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