Monoclonal antibodies to interferon alpha2 and hybridomas produc

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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530387, 530413, 435 7, 935104, 935108, 935110, 436548, C12N 512, C07K 1528, C12Q 100

Patent

active

049735560

ABSTRACT:
This invention relates to monoclonal antibodies to interferon alpha and to hybridomas producing such antibodies. These antibodies are preferably of the immunoglobulin subclass IgGl and preferably do not bind to alpha or alpha interferon. They can be used in assaying, purifying or isolating proteins, such as interferon alpha, for which they are specific.

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Shearer, M. et al. "Monoclonal Antibodies that Distinguish Between Subspecies of Human Interferon-.alpha. and that Detect Interferon Oligomers", J. Immunol. 133 (6): 3096-3101, Dec. 1984.
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Arnheiter, H. et al., Nature 294(5838):278-280 (1981), cited in Chem. Abstract CA96(15):12062.
Imai, et al., "Demonstration of Two Subtypes of Human Leuocyte Interferon (IFN-.alpha.) by Monoclonal Antibodies", J. Immunol., vol. 128, No. 6: 2824-2825 (1982).
Novick, et al., "Monoclonal Antibodies to Human.alpha.-Interferon and Their Use for Affinity Chromatography", J. Immunol., vol. 129, No. 5: 2244-2247 (1983).
Franke, et al., "Carboxyterminal Region of Hybrid Leukocyte Interferons Affects Antiviral Specificity", DNA, vol. 1, No. 3: 223-230 (1982).
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Staehelin et al., "Monoclonal Antibodies to Human Leukocyte Interferons: Their Use in Assay and Purification", Tex. Reports Biol. Med., vol. 41: 43-58 (1981).
Adolf et al., "Production of Monoclonal Antibodies to Human IFN-.alpha. and Their Use for Analysis of the Antigenic Composition of Various Natural Interferons", J. Cell. Physiol., vol. 2: 61-68 (1982).
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