Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-07-08
1989-08-08
Warden, Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 7, 436548, 436811, 530387, 935103, 935110, G01N 3353, C12N 500, C07K 1514
Patent
active
048552356
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to monoclonal antibodies for detecting an antigen present in human leukocytes, and to a process for producing the same.
BACKGROUND ART
It has become possible to produce monoclonal antibodies to T cells, B cells and their individual subsets of human lymphocytes by recently developed cell technological procedures, and the individual cell groups have been gradually clarified [Reinherz, E. L. et al: Cell 19, 821 (1980), Foon, K. A. et al: Blood 60, 1 (1982)].
That is, functional cell subsets of human lymphocytes have been much clarified by using monoclonal antibodies recognizing these subsets, and their abnormalities in various diseases have been gradually clarified.
DISCLOSURE OF INVENTION
As a result of extensive studies to find monoclonal antibodies applicable to diagnosis of various diseases of haemopoietic organs, etc., the present inventors have found monoclonal antibodies to the antigens present in leukocytes and to adult T cell leukemia (hereinafter referred to as "ATL"), and have established the present invention.
The present invention is explained in detail below:
The present invention provides monoclonal antibodies to the antigens present in leukocytes and ATL virus (hereinafter referred to as "ATLV").
Monoclonal antibodies of the present invention include those called Tpw40, Tp120, Ta60a, Ta60b, Ta60c, Ts60, Tsw32, TsA, TsB, Ts145, Lp95, Ls70, LsA, ATV19a, and ATV19b.
Ta60a, Ta60b and Ta60c characteristically fail to react with normal blood cells such as normal human peripheral blood T cell fraction, non-T(B) cell fraction, monocytes, granulocytes, thymocytes, etc but can react with the so-called activated T cells obtained by stimulating T cell groups by mitogens such as interleukin-2 [IL-2, Biotest Co. (West Germany)], phytohaemaglutinin [PHA, Difco Co. (USA)], concanavalin A [ConA, E.T Co. (USA)], pokeweed mitogen [PWM, Gibco Co. (USA)], etc., allo B cells, and the like (see Example 8). That is, they are characterized in that no response is observed under culturing conditions only with a culture liquor containing no mitogen as a control
Among the monoclonal antibodies of the present invention, Ta60a and Ta60b are positive to all the cases of ATL among those derived from T cells in the haemopoietic tumor cells failing to react with the cells derived from solid tumors, and are negative to substantially all the cases of malignant diseases derived from T cells other than ATL. This shows that the monoclonal antibodies of the present invention can be used for clearly distinguishing clinically hard-to-discriminate cases from one another
As for the tumor cells other than those derived from T cells, the monoclonal antibodies of the present invention characteristically undergo weakly positive reaction with some cases of B-CLL (B cell-type chronic lymphatic leukemia) and B-ML (B cell-type malignant lymphoma) and also undergo weakly positive reaction with some cases of AML (acute myeloid leukemia) derived from myelocytes
Tpw40 and Tpl20 react with most cells of T cell fraction of peripheral blood lymphocytes and detect the so-called T cells (panT).
Five antibodies of Ts60, Tsw32, TsA, TsB and Ts145 detect antigens present in some cells of T cell fraction and T subset antigens Ts60 has the same antigen molecular weight as that of Ta60, but has a different serological specificity. It does not react with activated T cells, but only with some of T cell strains.
Lp95 detects pan leukocyte antigen reacting with all the leukocytes, whereas Ls70 and LsA react with monocytes and some of other leukocytes and recognize leukocyte subset antigens.
Both Tpw40 and Tpl20 detect panT antigens Tpw40 reacts with relatively immature T cell-type acute lymphatic leukemia (T-ALL) and lymphoblast (LL) in the T cell tumor, whereas Tpl20 reacts with T.sub.2 lymphoma and ATL which are mature T cell tumors Furthermore, Tpl20 reacts with B cell-type chronic lymphatic leukemia (B-CLL) (Table 6).
Tsw32 reacts with T-ALL and LL, and TsA reacts with both these T cell tumors and also with
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Ohta Kazuo
Takahashi Toshitada
Ueda Ryuzo
Hoffer Florina B.
Takahashi Toshitada
Warden Robert J.
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