Monoclonal antibodies to human kininogen and methods of preparin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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43524027, 4351722, 435 7021, 935104, 935108, 935110, 530809, C07K 1504, C12N 500

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active

049084313

ABSTRACT:
Two novel cell lines, ATCC #HB-8963 and ATCC #HB-8964 produce monoclonal antibody to human kininogen. One of the antibodies specifically recognizes the heavy chain of high and low molecular weight kininogen (the later protein is identical to alpha cysteine protease inhibitor). The other antibody recognizes the light chain of high molecular weight kininogen. The hybridomas are formed by fusing spleen cells from immunized BALB/c AnSkh mice with P3X63Ag8 or SP2/0-Ag14 myeloma cells. Diagnostic, therapeutic and biochemical uses of the monoclonal antibodies are provided.

REFERENCES:
Bedi, G. S. et al. "Single Step Purification of `Kallikrein-Resistant` Kininogen from Rat Plasma Using Monoclonal-Antibody Immunoaffinity Chromatography", Preparative Biochemistry 15(3): 159-169, 1985.
Schmaier, A. H. et al. "High-Molecular Weight Kininogen: A Secreted Platelete Protein", J. Clin. Invest. 71: 1477-1489, 1983.
Berrettini, M. et al. "Immunological Studies of Human High Molecular Weight Kininogen (HWMK) Using Murine Monoclonal Antibodies (Mab)," Thrombosis and Hemostasis 54(1): 229, Jul. 14, 1985.
Goding, J. W. Monoclonal Antibodies: Principles and Practice, Academic Press, Inc., Orlando, 1983, pp. 56-97.
Muller-Esterl et al., "Purification and Properties of Human Low Molecular Weight Kininogen", Biochimica et Biophysica Acta, 706, 145-152 (1982).
Kerbiriou et al., J. Biol. Chem., 255, 3952-3958 (1980).

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