Monoclonal antibodies to cruciform DNA structure

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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53038821, 435 7021, 4351722, C12N 520, C12N 1502, C07K 1528, C12P 2108

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active

051926830

ABSTRACT:
The present invention relates to monoclonal antibodies of class IgG1 and IgM possessing among others the property to bind to stable heteroduplex cruciform DNA structures constructed from two DNA plasmids, to the hybrid cell lines producing such antibodies, to a method for producing the cell lines and to a method for enhancing DNA replication in a cell system containing cruciforms functioning as signals for the initiation of DNA replication.

REFERENCES:
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patent: 4868105 (1989-09-01), Urdea et al.
Frappier et al., Characterization of the Binding Specificity of Two Anticiform DNA Monoclonal Antibodies, The Journal of Biological Chemistry, vol. 264, No. 1, Jan. 5, 1989, pp. 334-341.
Frappier et al., Monoclonal Antibodies to Cruciform DNA Structures, Journal of Molecular Biology, vol. 193, No. 4, Feb. 20, 1987, pp. 751-758.
Nordheim et al., Negatively Supercoiled Plasmids Contain Left-Handed 2-DNA Segments as Detected by Specific Antibody Binding, Cell, vol. 31, 309-318, Dec. 1982 (Part 1).
Tenen et al., J. Virology SG(1):293-297, 1985.
Nobile et al., Intervirology 25:158-171 1986.
Eilat et al., J. Immunology Kipps et al., Handbook of Expt'l. Immunol. vol. 4, Weir Ed., Blackwell Sci. Publ. 1986.

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