Monoclonal antibodies to cell surface antigens of human teratoca

Chemistry: analytical and immunological testing – Involving production or treatment of antibody – Monoclonal antibody

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43524027, 530387, 935104, 935110, C12N 500, G01N 33534, G01N 33533, A61K 39395

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047628006

ABSTRACT:
Antibody-producing hybridoma cell lines made by fusion of NS/1 cells with spleen cells of mice after immunization with human teratocarcinoma cells are presented. Monoclonal antibodies from these cell lines recognize the K4, K2 and P12 antigenic systems and are thus useful in detecting and differentiating between normal and cancerous cells. These monoclonal antibodies are especially useful in pathologic analysis of human tumors, especially teratocarcinomas.

REFERENCES:
patent: 4563445 (1986-01-01), Feizi et al.
Blaineau et al, "The Glycosidic Antigen Recognized by a Novel Monoclonal Antibody. 75.12, is Developmentally Regulated", EMBO Journal 2(12) pp. 2217-2222 (1983).
Kannagi et al, "New Globoseries Glycosphingolipids in Human Teratocarcinoma Reactive with the Monoclonal Antibody Directed to a. . ." Journal of Biochemistry 258(14) pp. 8934-8942 (1983).
Miyauchi et al, "A New Fucosyl Antigen Expressed on Colon Adenocarcinoma and Embryonal Carcinoma Cells" Nature 299 pp. 168-169 (1982).
Gooi et al, "A Marker of Human Foetal Endoderm Defined by a Monoclonal Antibody involves Type I Blood Group Chains" Molecular Immunology 20(6) pp. 607-613 (1983).
Gooi et al, "Stage-Specific Embryonic Antigen Involves Alpha 1-3 Fucosylated Type 2 Blood Group Chains" Nature 292 pp. 156-158 (1981).
Gooi et al, "Marker of Peripheral Blood Granulocytes and Monocytes of Man Recognized by two Monoclonal Antibodies" European Journal of Immunology 13 pp. 306-312 (1983).
Kannagi et al, "Stage-Specific Embryonic Antigens (SSEA-3 and -4) are Epitopes of a Unique Globo-Series Ganglioside" EMBO Journal 2(12) pp. 2355-2361 (1983).

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