Monoclonal antibodies to ceftiofur

Details Monoclonal antibodies to ceftiofur Monoclonal antibodies to ceftiofur

1996-12-10

1999-06-01

Feisee, Lila

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Animal cell, per se, expressing immunoglobulin, antibody, or...

435 71, 435810, 5303881, 5303889, C12P 2108, C12N 512

Patent

active

059087811

ABSTRACT:
Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to the ceftiofur. These hybridomas may be obtained by using as an immunization agent or immunogen, desfuroyl ceftiofur which has been conjugated to an immunogenic carrier. Ceftiofur in biological samples may be detected and quantified by contacting the sample with the antibodies to form a ceftiofur/antibody immunocomplex when ceftiofur is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of ceftiofur.

REFERENCES:
Rose et al (ACS Symposium series, 621; 82-98), 1996.
Rose et al (Bioconjugate Chem, 1995, 6:529-535).
Kohler & Milstein (Nature, 1975, 256:495-497.
Kachab et al (J. Immunol Meth, 1992 , 147:33-41).
Jaglan et al (J. Assoc. Off Anal Chem, 1990, 73:26-30).
Current Protocols in Mol Bio, Ausubel et al, Eds, Green Pub Assoc & Wiley Interscience, 1992, pp. 11,15,2-4.
Harlow & Lane (Antibodies, Cold Spring Harbor Lab, 1988, p. 141).
Rose et al (J. Agri. Food Chem, 1992, 44: 622-627.

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