Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-03-02
1996-09-03
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
43524027, 435975, 436518, 436548, 5303881, G01N 3353, G01N 33543, C07K 1618, C12N 520
Patent
active
055522955
ABSTRACT:
Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind bovine haptoglobin, either in its native state or bound to hemoglobin and/or albumin. These hybridomas may be obtained by using as an immunization agent or immunogen, bovine haptoglobin that has been complexed to hemoglobin and optionally albumin. Total bovine haptoglobin, including both native and bound to hemoglobin and/or albumin, present in biological samples may be detected and quantified by contacting the sample with the antibodies to form a bovine haptoglobin/antibody immunocomplex when the bovine haptoglobin is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of bovine haptoglobin.
REFERENCES:
Busby and Travis, 1978, Comp. Biochem Phys. vol. 60B pp. 389-396 "Structure and Evolution of Artiodactyla Haptoglobins".
Nakamura et al, Enzyme Immuroassays: Heterogeneous and Homogeneous system: in Handbook of Experimental Immunology. 1986 Blackwell Scientific pub. vol. 1, pp. 27.1-27.2.
"Immunogenicity and Antigen Structure in: Fundamental Immunology" Paul. ed. 1989 Raven Press, NY. pp. 177-178.
Katnik et al, 1989, Hybridoma V 8 No. 5 pp. 551-560. "Monoclonal Antibodies Against Human Haptoglobin".
Eckersall and Connor, 1990 comp. Biochem. Physiol. vol. 96B No 2 pp. 309-314. "Plasma Haptoglobin in cattle . . . ".
Campbell, "Monoclonal Antibody and Immunosensor Technology in: Laboratory techniques . . . " 1991, Elsevier Science Pub. NY, vol. 23, p. 45.
Katnik et al., 1993, Archivum Immunological and Therapial Experimentalis vol. 41 pp. 105-109. "Quantitation of Human Heptoglobin by Elisa System . . . ".
DeLoach John R.
Sheffield Cynthia L.
Stanker Larry H.
Deck Randall E.
Duffy Patricia A.
Fado John D.
Scheiner Toni R.
Silverstein M. Howard
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