Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...
Patent
1996-11-18
1999-01-05
Huff, Sheela
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Animal cell, per se, expressing immunoglobulin, antibody, or...
5303877, 5303888, 435 723, C12N 506, C12N 516, G01N 33574, C07K 1600
Patent
active
058561820
ABSTRACT:
Monoclonal antibodies have been developed which specifically bind the PSA-ACT complex without significant cross-reactivity with PSA, ACT or CG-ACT. The antibodies were generated using a unique immunogen comprising a PSA-ACT complex having bound to monoclonal antibodies against PSA and monoclonal antibodies against ACT. It is theorized that the antibodies against PSA and ACT block immunodominant sites on the PSA-ACT complex. The antibodies generated against the unique immunogen specifically bind the PSA-ACT complex, but have no significant cross-reactivity with either PSA or ACT. The monoclonal antibodies of the present invention can be used in immunoassays to specifically detect PSA-ACT in a sample.
REFERENCES:
patent: 5501983 (1996-03-01), Lilja et al.
Sandhu, J.S. "Protein Engineering of Antibodies" in Critical Reviews in Biotechnology, vol. 12, issues 5 and 6, pp. 437-445, 1992.
Paul, W. E. "Fundamental Immunology" Second Edition, Raven Press, NY, pp. 177-181, 1989.
Schlom, J. "Monoclonal Antibodies: They're more and less than you think" in Molecular Foundations of Oncology, ed. Samual Broder, pp. 95-134, 1991.
The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Specific Antigen-.alpha..sub.1 -Antichymotrypsin Complexes in Serum, W G. Wood, et al., Eur. J. Clin. Chem. Clin. Biochem. vol. 29, pp. 787-794, 1991.
A Complex between Prostate-Specific Antigen and .alpha..sub.1 -Antichymotrypsin Is the Major Form of Prostate-specific Antigen in Serum of Patients with Prostate Cancer: Assay of the Complex Improves Clinical Sensitivity for Cancer. U. Stenman, et al., Cancer Research 51, 222-226 Jan. 1, 1991.
Serum Prostate Specific Antigen Complexed to .alpha.1-Antichymotrypsin As An Indicator Of Prostate Cancer, Anders Christensson, et al., The Journal of Urology, vol. 150, 100-105, Jul. 1993.
Correlation of Serum Concentrations of PSA-ACT Complex With Total PSA in Random and Serial Specimens From Patients With BPH and Prostate Cancer, J. T. Wu, et al. Journal of Clin. Lab. Anal. 9:15-24 (1995).
Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors, A. Christensson, et al., Eur. J. Biochem., 194, 755-763, 1990.
Purification of PSA-ACT Complex: Characterization of PSA-ACT Complex by Various Chromatographic Procedures, Jame T. Wu, et al., Journal of Clin. Lab. Analysis 9:25-31 (1995).
Free, Complexed And Total Serum Prostate Specific Antigen: The Establishment Of Appropriate Reference Ranges For Their Concentrations And Ratios, Joseph E. Oesterling, et al., The Journal of Urology, vol. 154, 1090-1095, Sep., 1995.
Characterization Of Five Different Epitopes Of Anti-PSA Monoclonal Antibodies, Tang J. Wang, et al., Clin. Chem., vol. 41, No. 6, 1995.
Prostate-Specific Antigen in Serum Occurs Predominantly in Complex with .alpha..sub.1 -Antichymotrypsin, Hans Lilja, et al. Clin. Chem. 37/9, 1618-1625 (1991).
Double-Label Time-Resolved Immunofluorometric Assay of Prostate-Specific Antigen and of Its Complex with .alpha..sub.1 -Antichymotrypsin, Jari Leinonen, et al., Clin. Chem., Vol. 39, No. 10, 1993.
Improved Separation Between Normals, Benign Prostatic Hyperplasia (BPH), and Carcinoma of the Prostate (CAP) by Measuring Free (F), Complexed (C), and Total Concentrations (T) of Prostate Specific Antigen (PSA), Hans Lilja, et al., Journal of Urology, 151, 400A, 1994.
Kuus-Reichel Kristine
Linton Harry Jay
Payne Janice K.
Wang Tang J.
Beckman Coulter Inc.
Eyler Yvonne
Huff Sheela
Kivinski Margaret A.
May William H.
LandOfFree
Monoclonal antibodies specific for the PSA-ACT complex does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Monoclonal antibodies specific for the PSA-ACT complex, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monoclonal antibodies specific for the PSA-ACT complex will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-861587