Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1985-03-01
1986-09-16
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351722, 435240, 436548, 935104, 935110, C12P 2100, C12N 1500, C12N 500, G01N 3354
Patent
active
046122826
ABSTRACT:
Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.
REFERENCES:
Goldsby et al., "Production of Specific Antibody without Specific Immunization", Current Topics in Microbiology and Immunology, vol. 81, pp. 149-151 (1979).
Schlom et al, "Generation of Human Monoclonal Antibodies Reactive with Human Mammary Carcinoma Cells", Proceedings of the National Academy of Sciences, 77(11), pp. 6841-6845 (1980).
Kohler et al, "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity", Nature, 256, pp. 495-497 (1975).
"High Frequencies of Antigen Specific Hybridomas: Dependence on Immunization Parameters and Prediction by Spleen Cell", Journal of Immunological Methods, 32, pp. 297-304 (1980).
Austin Faye
Colcher David
Hand Patricia H.
Nuti Marianna
Schlom Jeffrey
Roberts, Jr. John S.
Tarcza John Edward
The United States of America as represented by the Secretary of
Wiseman Thomas G.
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