Monoclonal antibodies, hybridomas, improved method for...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C435S007240, C435S007500, C435S007920, C435S028000, C435S070210, C435S336000, C435S337000, C435S343000, C436S518000, C436S531000, C436S548000, C436S164000, C530S388240, C530S388250, C530S388700, C530S389300, C530S389600, C530S391100

Reexamination Certificate

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07915004

ABSTRACT:
Method for determining the level of PTX3 protein in a sample of a biological fluid; hybridoma capable of producing a rat anti-PTX3 monoclonal antibody where said hybridoma is selected from the group comprising MNB10 and Pen-3; specific anti-PTX3 rat monoclonal antibody selected from the monoclonal antibodies produced by the hybridomas MNB10 and Pen-3; kit for determining the level of PTX3 protein in a biological fluid wherein the said kit includes a rat anti-PTX3 monoclonal antibody.

REFERENCES:
patent: 6537745 (2003-03-01), Chien et al.
patent: 2004/0137544 (2004-07-01), Latini et al.
Boscato et al., 1986. Incidence and specificity of interference in two-site immunoassays. Clinical Chemistry 32: 1491-1495.
Harlow et al., 1988. Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor. p. 593.
Hellström et al., 1985. In Monoclonal Antibodies for Cancer Detection and Therapy (Baldwin et al, eds.), Academic Press, London. p. 20.
Muller et al., 2001. Circulating levels of the long pentraxin PTX3 correlate with severity of infection in critically ill patients. Critical Care Medicine 29: 1404-1407.
Vouret-Craviari et al., 1997. Expression of a long pentraxin, PTX3, by monocytes exposed to the Mycobacterial cell wall component lipoarabinomannan. Infection and Immunity 65: 1345-1350.
Fazzini, Fausto et al., “PTX3 in Small-Vessel Vasculitides: An Independent indicator of disease activity produced at sites of inflammation”, Arthritis & Rheumatism, vol. 44, No. 12, pp. 2841-2850, 2001.
Breviario, Ferruccio et al., “Cloning of a new gene related to C-reactive protein and serum amyloid P component”, The Journal of Biological Chemistry, vol. 267, No. 31, pp. 22190-22197, 1992.
Alles, Victor Vidal et al., “Inducible Expression of PTX3, a New Member of the Pentraxin Family, in Human Mononuclear Phagocytes”, Blood, vol. 84, No. 10, pp. 3483-3493, 1994.
Bottazzi, Barbara et al., “Multimer Formation and Ligand Recognition by the Long Pentraxin PTX3—Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component”, The Journal of Biological Chemistry, vol. 272, No. 52, pp. 32817-32823, 1997.
Peri, Giuseppe et al., “PTX3, A Prototypical Long Pentraxin, Is an Early Indicator of Acute Myocardial Infarction in Humans”, Circulation, vol. 102, pp. 636-641, 2000.
Latini R. et al., Prognostic significance of the long pentraxin PTX3 in acute myocardial Infarction: comparison with C-reactive protein, NT-proBNP and troponin T. abstract 3091, Supplement IV Circulation , vol. 108, No. 17, p. 680, 2003.

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