Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1997-08-08
1999-01-12
Chin, Christopher L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
436547, 436548, 436808, 530380, 5303881, 53038815, 5303879, 530808, 530809, 530829, G01N 3353
Patent
active
058592032
ABSTRACT:
The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.
REFERENCES:
M.G. Buckley et al., "Immunoreactive N-Terminal Pro-Atrial Natriuretic Peptide in Human Plasma: Plasma Levels and Comparisons with .alpha.-Human Atrial Natriuretic Peptide in Normal Subjects, Patients with Essential Hypertension, Cardiac Transplant and Chronic Renal Failure," Clinical Science, 77, pp. 573-579 (1989).
M.G. Buckley et al., "Concentrations of N-Terminal ProANP in Human Plasma: Evidence for ProANP (1-98) As The Circulating Form," Clinica Chimica Acta, 191, pp. 1-14 (1990).
M.G. Buckley et al., "N-Terminal Pro Atrial Natriuretic Peptide in Human Plasma," American J. Hypertension, 3, pp. 933-935 (1990).
E. Ishikawa et al., Enzyme Immunoassay, 3rd Ed., pp. 75-126 (1987).
H. Itoh et al., ".gamma.-Atrial Natriuretic Polypeptide (.gamma.ANP)-Derived Peptides in Human Plasma: Cosecretion of N-Terminal .gamma.ANP Fragment and .alpha.ANP," J. Clinical Endocrinology and Metabolism, 67, pp. 429-437 (1988).
B.F. Lindberg et al., "Radio-immunoassay of Atrial Natriuretic Peptide (ANP) and Characterization of ANP Immunoreactivity in Human Plasma and Atrial Tissue," Scand. J. Clin. Lab. Invest., 52, pp. 447-456 (1992).
M. Mukoyama et al., "Preparation of Monoclonal Antibodies Against Atrial Natriuretic Polypeptide Precursor and Application to Highly Sensitive Sandwich Enzyme Immunoassay," J. Hypertension, 6 (suppl 4), pp. S320-S322 (1988).
J.A. Sundsfjord et al., "Identification and Plasma Concentrations of the N-Terminal Fragment of Proatrial Natriuretic Factor in Man," J. Clinical Endocrinology and Metabolism, 66, pp. 605-610 (1988).
T. Tsuji et al., "Stability of Human Atrial Natriuretic Peptide in Blood Samples," Clinica Chimica Acta, 225, pp. 171-177 (1994).
Asada Hidehisa
Dohi Keiji
Fukunaga Takahiro
Numata Yoshito
Taniguchi Yasushi
Chin Christopher L.
Haley, Jr. James F.
Nguyen Bao-Thuy L.
Shionogi & Co. Ltd.
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