Monoclonal antibodies for DNA-RNA hybrid complexes and their use

Details Monoclonal antibodies for DNA-RNA hybrid complexes and their use Monoclonal antibodies for DNA-RNA hybrid complexes and their use

1982-12-29

1988-03-22

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435 68, 4351722, 43524027, 435188, 435810, 436501, 436503, 436504, 436518, 436527, 436543, 436548, 436804, 436808, 530387, 530389, 530809, 935104, 935108, 935110, C12Q 168, G01N 33577, C07K 1504

Patent

active

047328479

ABSTRACT:
Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.

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