Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1993-06-10
1995-03-28
Bidwell, Carol E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
435 722, 43524027, 530822, C07K 1528, G01N 33569
Patent
active
054018315
ABSTRACT:
A method to prepare a monoclonal antibody (mAb) specifically immunoreactive with an epitope of the Gal/GalNAc lectin of a pathogenic or nonpathogenic E. histolytica, or the 70 kd subunit thereof, is provided. This method comprises culturing an immortalized cell line capable of secreting the mAbs, where the cell line is obtained by immortalizing antibody-producing cells from a mammal immunized with a purified 170 kd subunit, or with purified Gal/GalNAc lectin of a pathogenic E. histolytica. The supernatants of the cells are screened for the presence or absence of antibodies specific for the subunit or lectin from a pathogenic E. histolytica and from a pathogenic E. histolytica, so as to determine the pathogen
onpathogen specificity of the monoclonal antibody. Antibodies produced by this method and epitopes with which they react on pathogenic and/or nonpathogenic E. histolytica also are disclosed.
REFERENCES:
patent: 5004608 (1991-04-01), Ravdin et al.
patent: 5272058 (1993-12-01), Petri et al.
Kohler et al (1975) "Continuous cultures of fused cells . . . " Nature 256:495-497.
Petri et al (1987) "Recognition of the Galactose-. . . " Infect. & Immun. 55:2327-2331.
Ravdin et al., "Relationship of Free Intracellular Calcium to the Cytolytic Activity of Entamoeba histolytica", Infect. Immun. 56:1505-1512 (1988).
Petri, Jr. William A.
Ravdin Jonathan I.
Bidwell Carol E.
University of Virginia Patents Foundation
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