Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1986-01-03
1989-06-27
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 5, 435 7, 435 68, 435810, 4351722, 436548, 436808, 436809, 436811, 530387, 530808, 530809, 935 95, 935100, 935104, 935106, 935107, 935108, 935110, G01N 33577, C12N 1500
Patent
active
048430110
ABSTRACT:
Monoclonal antibodies, and hybridoma cell lines for their production, that bind with a high degree of specificity proteins associated with HTLV-III virus are presently disclosed. In particular, transmembrane envelope glycoprotein gp41 (41,000 dalton molecular size), major core antigen p24 (24,000 dalton molecular size), and p17 protein (17,000 dalton molecular size) are disclosed. The proteins to which the present monoclonal antibodies respond are essentially antigenically distinct from HTLV-I and HTLV-II. SVM-16 is an IgM monoclonal antibody, SVM-23 is an IgG.sub.2 monoclonal antibody, and SVM-26 is an IgG.sub.1 monoclonal antibody, all of which bind to p24. SVM-25 is an IgG.sub.1 monoclonal antibody binding gp41, and SVM-33 is an IgG.sub.1 monoclonal antibody binding p17. All the monoclonal antibodies of the present invention are produced in hybridoma cells prepared by fusing myeloma cells with spleen cells from mammals, such as mice, immunized with lysates of purified virus.
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di Marzo Veronese Fulvia
Gallo Robert C.
Sarngadharan Mangalasseril G.
Akzo N.V.
Blackstone William M.
Nucker Christine M.
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