Monoclonal antibodies and vaccine development directed to human

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Cancer cell or component thereof

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4241841, 4241931, 514 8, 514 23, 530395, A61K 3814, A61K 3900, A61K 3939, C07K 1482

Patent

active

056608349

ABSTRACT:
A vaccine and method to prevent growth and replication of cancer cells that express a core structure of a mucin-type glycoprotein is disclosed. The vaccine comprises: (a) a pharmaceutically effective amount of an antigen comprising a purified mucin-type glycoprotein or a chemically synthesized mucin-type glycoprotein carbohydrate determinant conjugated to a carrier peptide or macromolecule, wherein said mucin-type glycoprotein expresses or carries the core structure of a mucin-type glycoprotein expressed on said cancer cells; and (b) a pharmaceutically acceptable carrier including natural or synthetic adjuvants. The method comprises administering the above-described vaccine to a host. A medicament and method for treating cancer wherein the cancer cells express a core structure of a mucin-type glycoprotein is disclosed. The medicament comprises: (a) a pharmaceutically effective amount of an anti-cancer antibody or fragment comprising a binding site thereof, said anti-cancer antibody having been produced against a purified core structure of a mucin-type glycoprotein; and (b) a pharmaceutically acceptable carrier, diluent or excipient. The method comprises administering the above-described medicament to a host.

REFERENCES:
Basic and Clinical Immunology (1980), pp. 304-312.
Hakomori, S., Ann. Rev. Immunol 2:103-126 (1984), "Tumor-associated carbohydrate antigens".
Lan, M. S., et al. J. Biol. Chem 262:12863-12870 (1987), "Molecular Characterization of a mucin-type antigen associated with a human pancreatic cancer."
Magnani, J. L., et al. Cancer Res. 43:5489-92 (1983), "Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by MAb 19-9 in the sera of patients as a mucin."
Sharon, N. et al. Scientific American, Jan. 1993. pp. 82-89. "Carbohydrates in cell recognition."
Boon, T. Sci. Am. Mar. 1993. pp. 82-89. Teaching the Immune system to fight cancer.

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