Monoclonal antibodies and vaccine development directed to human

Details Monoclonal antibodies and vaccine development directed to human Monoclonal antibodies and vaccine development directed to human

1991-12-16

1993-07-20

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

530808, 5303875, 53038885, C12N 518, C07K 1528

Patent

active

052292894

ABSTRACT:
A method of producing monoclonal antibodies that bind to human cancer-associated mucin-type glycoprotein antigens comprising: (1) immunizing a host with a core structure of a mucin-type glycoprotein: (2) fusing splenocytes from said immunized host with myeloma cells to form hybridoma cells; (3) culturing said hybridoma cells on selective medium; (4) selecting hybridoma cells surviving step (3) that secrete antibody that binds to said core structure of a mucin-type glycoprotein; (5) cloning said selected hybridoma cells from step (4); (6) culturing said cloned hybridoma cells; and (7) recovering said antibody. Hybridomas and monoclonal antibodies produced by the above-described method. Methods of passive and active immunization employing the monoclonal antibodies and mucin-type glycoproteins or synthetic oligosaccharide-carrier conjugates.

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