Monoclonal antibodies and hybridomas specific for green-oat phyt

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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5303881, 5303911, 530370, 4351722, 435 7021, C12N 512, C12P 2108, C07K 1528

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active

052486079

ABSTRACT:
The present invention provides a novel method to purify green-plant phytochrome; the production of hybridoma cell lines secreting monoclonal antibodies specific for green-plant phytochrome and that do not cross-react significantly with etiolated-plant phytochrome; a partial amino acid sequence for an immunoaffinity-purified green-plant phytochrome; oligonucleotide probes for cloning green-phytochrome genes from plant genomic DNA and cDNA libraries; and cloned green-phytochrome genes and cDNA.

REFERENCES:
Cordonnier, et al. (1988) J. Cell Biochem Suppl O (12 Part C) p. 169, 1988.
Konomi et al. (1987) Plant Cell Physiol 28: 1443-1451.
Shimazaki et al. (1985) Planta 164: 333-344.
Pratt et al. (1987) in Phytochrome and Photoregulation in Plants, M. Furuya, ed., Academic Press, Tokyo, pp. 83-94.
Tokuhisa et al., Planta, 164:321-332 (1985).
Cordonnier et al., Biochemistry, 25:7657-7666 (1986).

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