Monoclonal antibodies against Mycoplasma pneumoniae, hybridomas

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 794, 435 7021, 435340, 435975, 436547, 436548, 5303881, 5303882, 5303884, G01N 3353, C07K 1600, C12P 2108

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056416385

ABSTRACT:
The invention relates to monoclonal antibodies and fragments thereof which are specific for Mycoplasma pneumoniae P2 protein and have a cross-reactivity of 1% or less with five other species of the genus Mycoplasma and other pathogen species of the concomitant flora, and to methods for the preparation of the monoclonal antibodies according to the invention. The invention furthermore relates to hybridomas which produce the antibodies according to the invention, and to methods for the preparation thereof. Finally, the invention relates to the use of the monoclonal antibodies according to the invention for detecting Mycoplasma pneumoniae in a sample.

REFERENCES:
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patent: 5084561 (1992-01-01), Bredt et al.
Hirschberg et al, "ELISA for detection of Mycoplasma pneumoniae antigens using monoclonal antibodies", APMIS, 99(5): 475-481 (May 1991).
Hirschberg et al, "Demonstration of Membrane Association and Surface Location of Mycoplasma pneumoniae Antigens Using Monoclonal Antibodies", J. Gen Microbiol., 135:613-621 (1989).
Gerstenecker et al, "Topological mapping of the P1-adhesion of Mycoplasma pneumoniae with adherence-inhibiting monoclonal antibodies", J. Gen. Microbiol., 136: 471-476 (1990).
Harlow et al. Antibodies, A Laboratory Manual, pp. 141-150, 231-239 Cold Spring Harbor Laboratory (1988).
Nachweis Von Spezifischen Antikorpern Und Antigenen Bei Patienten Mit Mycoplasma Pneumoniae-Infektionen, E. Jacobs et al., Diagnostik Von Infektionskrankheiten, 1:84-105 (1987).
Laboratory Diagnosis of Mycoplasma Pneumoniae Infection, T. W. Kok et al., Epidem. Inf. 101:669-684 (1988).
Tissue Cultures and Mycoplasmas, L. Hayflick, Texas Reports on Biology and Medicine 23:s 285-303 (1965).

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