Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1996-01-11
1999-06-08
Hutzell, Paula K.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
530300, 530326, 530350, 5303871, 514 2, 435 71, 435 72, 435 792, C07K 1400, A61K 3800, A61K 3816
Patent
active
059105682
ABSTRACT:
A purified polypeptide which provides for initial binding of sperm to oocyte investments and has an active amino acid sequence of SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-A sn -Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-P ro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro -Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys); or SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-S er-Asn-Lys-Ile-Pro-Glu-Leu -Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-P ro-Gln-Asp-Gly-Pro-Arg-Ser-Lys-Pro-Gln -Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys); or the shorter but biologically active SEQ ID NO:1 and SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, where X is Thr or Pro). The polypeptide is useful in improving sperm-egg binding by fresh sperm, restoring sperm-egg binding following a cryopreservation cycle, enhancing fertilizing potential, and producing antibodies to determine fertilizing potential of sperm, determining sperm-binding sites on egg investments, and contraception.
REFERENCES:
patent: 3718740 (1973-02-01), Hafs et al.
patent: 4283419 (1981-08-01), Voronkov et al.
patent: 4683213 (1987-07-01), Ax
patent: 4767703 (1988-08-01), Ax et al.
patent: 4879213 (1989-11-01), Fox et al.
patent: 4945044 (1990-07-01), Huszar
patent: 5175148 (1992-12-01), O'Rand et al.
Cramer et al (Biology of Reproduction May 1994) vol. 50 (5) Abstract 293.
Lazar et al (1988) Mol. Cell. Biol. vol. 8(3), 1247-1252.
Burgess et al (1990) J. Cell. Biol. vol. 111, 2129-2138.
Jean et al., "Increased zona-binding ability after incubation of spermatozoa with proteins extracted from spermatozoa of fertile semen", Journal of Reproduction and Fertility, vol. 105, pp. 43-48 (1995).
Killian et al., "Fertility-Associated Proteins in Holstein Bull Seminal Plasma", Biology of Reproduction, vol. 49, pp. 1202-1207 (1993).
Saling, "Mammalian sperm interaction with extracellular matrices of the egg", Oxford Reviews of Reproductive Biology, vol. 11, pp. 339-388 (1989).
Wassarman, "Regulation of Mammalian Fertilization by Gamete Adhesion Molecules", Schering Foundation Workshop 4, Spermatogenesis-Fertilization-Contraception, pp. 345-366 (1992).
Sylvester et al., "Sulfated Glycoprotein-1 (Saposin Precursor) in the Reproductive Tract of the Male Rat", Biology of Reproduction, vol. 41, pp. 941-948 (1989).
Collard et al., "Biosynthesis and Molecular Cloning of Sulfated Glycoprotein 1 Secreted by Rat Sertoli Cells: Sequence Similarity with the 70-Kilodalton Precursor to Sulfatide/G .sub.M1 Activator", Biochemistry, vol. 27, pp. 4557-4564 (1988).
Hiraiwa et al., "Isolation, Characterization, and Proteolysis of Human Prosaposin, the Precursor of Saposins (Sphingolipid Activator Proteins)", Archives of Biochemistry and Biophysics, vol. 304, No. 1, pp. 110-116 (1993).
Tsuda et al., "The Primary Structure of Mouse Saposin", Biochemical and Biophysical Research Communications, vol. 184, No. 3, pp. 1266-1272 (1992).
Kishimoto et al., "Saposins: structure, function, distribution, and molecular genetices", Journal of Lipid Research, vol. 33, pp. 1255-1267 (1992).
O'Brien et al., "Saposin proteins: structure, function, and role in human lysosomal storage disorders", The FASEB Journal, vol. 5, pp. 301-308 (1991).
Bookbinder et al., "Tissue--and Species-Specific Expression of sp56, a Mouse Sperm Fertilization Protein", Science, vol. 269, pp. 86-88 (1995).
Echeverria et al., "Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to ferlitlize in vivo and in vitro", J. Reprod. Fert., vol. 71, pp. 433-437 (1984).
Igdoura et al., "Sulfated Glycoprotein-2 Synthesized by Nonciliated Cells of the Efferent Ducts is Targeted to the Lysosomal Compartment," Microscopy Research and Technique, pp. 468-480 (1984).
Igdoura et al., "Role of Sulfated Glycoprotein-1 (SGP-1) in the Disposal of Residual Bodies by Sertoli Cells of the Rat," Molecular Reproduction and Development, 40. pp. 91-102 (1995).
Rosenthal et al., "Hormonal Regulation of Sulfated Glycoprotein-1 Synthesis by Nonciliated Cells of the Efferent Ducts of Adult Rats," Molecular Reproduction and Development, 40 pp. 69-83 (1995).
Hermo et al., "Immunocytochemical Localization of Sulfated Glycoprotein-1 (SGP-1) and Identification of Its Transcripts in Epithelial Cells of the Extratesticular Duct System of the Rat," The Anatomical Record, 232, pp. 401-422 (1992).
Leonova et al., "Proteolytic Processing Patterns of Prosaposin in Insect and Mammalian Cells," The Journal of Biological Chemistry, vol. 271, No. 29, pp. 17312-17320 (1996).
Zafian et al., "The Retention of .sup.125 I-Labeled Plasma Membrane Proteins in Bovine Spermatozoa Cryopreserved in Egg-Yolk Citrate Extender," Gamete Research pp. 367-377 (1985).
Barbato Guy F.
Cramer Palmer G.
Hammerstedt Roy H.
Hutzell Paula K.
Masood Khalid
Monahan Thomas J.
The Penn State Research Foundation
LandOfFree
Molecule involved in binding of sperm to egg surfaces and proced does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Molecule involved in binding of sperm to egg surfaces and proced, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecule involved in binding of sperm to egg surfaces and proced will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1683875