Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-03-21
1998-10-27
Guzo, David
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 435325, 435348, 4352523, 4352542, 435419, 536 231, 536 241, C12N 510, C12N 1586, C12N 1588, C07H 2104
Patent
active
058277056
ABSTRACT:
The invention provides a nuclear targeting molecule having a nucleic acid sequence which consists of SEQ ID NO:1 or a nuclear targeting portion of SEQ ID NO:1. The invention further provides a plasmid for targeting a DNA molecule into the nucleus of a host cell. The plasmid comprises the nuclear targeting molecule (having SEQ ID NO:1 or a nuclear targeting portion of SEQ ID NO:1) and a DNA molecule to be targeted to a nucleus. This plasmid of the subject invention can be introduced into various host cells, and the nuclear targeting molecule will target the DNA molecule to the nucleus of the host cell. Thus, the invention further provides a method of targeting a DNA molecule into the nucleus of a host cell. The method comprises providing a plasmid (the plasmid comprising the nuclear targeting molecule and the DNA molecule to be targeted) and introducing the plasmid into the cytoplasm of the host cell. In this method, the nuclear targeting molecule targets the DNA molecule into the nucleus of the host cell.
REFERENCES:
Ishii, N., et al., J Virol 68 (12) :8209-8216 (Dec. 1994).
Feldherr, C., et al., Exp Cell Res 213 (1) :164-171 (Jul. 1994).
Dean, D.A., and Kasamatsu, H., J Biol Chem 269(7) :4910-4916 (Feb. 18, 1994).
Xue, Z., et al., Eur J Cell Biol 62(1) :13-21 (Oct. 1993).
Feldherr, C.M., et al., Proc Natl Acad Sci USA 89 (22) :11002-11005 (Nov. 15, 1992).
Yoneda, Y., et al., Exp Cell Res 201(2) :313-320 (Aug. 1992).
Lassner, M.W., et al., Plant Mol Biol 17(2) :229-234 (Aug. 1991).
Rihs, H.P. , et al., EMBO J 10(3) :633-639 (Mar. 1991).
Clever, J., and Kasamatsu, H., Virology 181(1) :78-90 (Mar. 1991).
Ono, T., e al., Neurosci Lett 117(3) :259-263 (Sep. 18, 1990).
Gharakhanian, E., and Kasamatsu, H., Virology 178(1) :62-71 (Sep. 1990).
Lobl, T.J., et al., Biopolymers 29(1): 197-203 (Jan. 1990).
Wychowski, C., et al., EMBO J 5(10) :2569-2576 (Oct. 1986).
Graessmann, M., et al., Nucl Acids Res 17(16) :6603-6612 (1989).
Dean, D.A., Abstract #313 of the Keystone Symposia on Molecular and Cellular Biology, Taos, New Mexico, Feb. 4-10, 1996.
Braman, Esq. Susan J.
Guzo David
South Alabama Medical Science Foundation
LandOfFree
Molecule and method for importing DNA into a nucleus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Molecule and method for importing DNA into a nucleus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecule and method for importing DNA into a nucleus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1613168