Molecular methods for detecting guar gum additions to locust...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S023100, C536S024300

Reexamination Certificate

active

07037653

ABSTRACT:
The invention concerns methods for detecting guar gum alone or in mixtures of guar gum and locust bean gum. The invention also concerns methods for extracting, amplifying, and detecting DNA of guar and locust bean gums and the mixtures thereof. DNAs of plants from which guar and locust bean gums are extracted are amplified by means of polymerase chain reaction (PCR) using conserved initiators. Differences in the sequences of the amplification products obtained from these two plants enable their differentiation, the identification of guar DNA in mixtures of guar and locust bean gum mixtures and the design of guar-specific PCR initiators which detect guar only in mixtures of guar and locust bean gum.

REFERENCES:
patent: WO 98/14607 (1998-04-01), None
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Accession No. AF274687, EMBL/GenBank/DDBJ database, Barker, N.P., et al.: “Generic relationships in the tribe Indigoferae (Leguminosae: Papilionoideae) based on sequence data and morphology” Jun. 5, 2000.
Dlauchy, Denes et al., “Restriction Enzyme Analysis of PCR Amplified rDNA as a Taxonomic Tool in Yeast Identification.” 1999 Syst. Appl. Microbiol. 22 (3), 445-453.
Dawkins, N.L. Studies on the extractability, structural and physicochemical properties or oat gum/beta. glucan. 1994 Dissertation International Abstract, 55 (4) 1241-B, No. DA9423975.
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