Molecular mechanisms for gene containment in plants

Details Molecular mechanisms for gene containment in plants Molecular mechanisms for gene containment in plants

2007-07-03

2007-07-03

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of using a plant or plant part in a breeding process...

Method of breeding using gametophyte control

C800S278000, C800S287000, C800S288000, C800S290000, C435S199000, C435S320100, C435S419000, C536S023700, C536S024100

Reexamination Certificate

active

10644664

ABSTRACT:
The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

REFERENCES:
Kriete et al. The Plant Journal 9(6): 809-818 (1996).
Williams, M. Trends in Biotechnology 13: 344-349 (Sep. 1995).
Kuvshinov et al. (2004) Barnase gene inserted in the intron of GUS . . . Plant Science 167: 173-182.
Edgell, D. et al. Barriers to Intron Promiscuity in Bacteria. (2000) J. Bacteriology 1282(19) 5281-5289.
Schernthaner J. (2003) Control of seed germination in transgenic plants . . . PNAS vol. 100 No. 11. pp. 685-6859.
Gressel 1999: Tandem constructs: preventing the rise of superweeds. TIBTECH vol. 17 pp. 361-366.
Daniell H. (2002) Molecular strategies for gene containment in transgenic crops. Nature biotec, 20 pp. 581-586.

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