Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-09-26
2000-11-21
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 235, 536 2433, C12Q 168, C07H 2104
Patent
active
061500968
ABSTRACT:
The present invention relates to an arbitrarily primed PCR-based method to identify genetic marker associated with a pathology, which comprises the steps of: a) collecting heterogeneous nucleic acid samples from diseased and healthy tissues; b) determining quantities of the nucleic acid pools by specific amplification of a fragment of glyceraldehyde-phosphate dehydrogenase (GAPDH) mRNA; c) amplifying nucleic acid pools of step b) using non-specific sense and antisense primers to obtain clear patterns of nucleic acid sequences; and d) subjecting amplified nucleic acid sequences to gel electrophoresis to obtain fingerprints of both diseased and healthy, wherein bands predominantly associated with diseased tissues are markers for the pathology. The present invention also relates to a RNA expression marker for the diagnosis of Crohn's disease, which comprises a 3.1 kb RNA sequence, and uses thereof.
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LaFontaine Daniel
Mercure Stephane
Perreault Jean-Pierre
Arthur Lisa B.
Murphy Kevin P.
Souaya Jehanne
Universite de Sherbrooke
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