Molecular markers

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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07101668

ABSTRACT:
Nucleic acid based methods for detecting the presence ofE. coliorShigellaor related microorganisms in a sample using one or moreE. coliorShigellaspecies specific nucleotide sequences are disclosed. More particularly the identification of molecules capable of binding or otherwise facilitating abnormal cell growth or abnormal physiology such as found in cancer or cellular instability is described. Further, molecular probes for performing the nucleic-acid based methods and methods of testing and selecting nucleic acid sequences suitable for same are provided. The methods and polynucleotides are useful inter alia in the testing of food and water samples, for testing for genetic and cellular instability, and for testing benign, pre-neoplastic and neoplastic disease in asymptomatic or symptomatic colorectal or gastric cancer patients or those at risk of the aforementioned conditions or those infected by Escherichieae and with other diseases or conditions.

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Swidsinski, et al., “Association Between IntraepithelialEscherichia coliand Colorectal Cancer,”Gastroenterology, 1998, vol. 115, pp. 281-286.

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