Molecular marker

Details Molecular marker Molecular marker

2003-10-31

2009-08-04

Haddad, Maher M (Department: 1644)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S381000

Reexamination Certificate

active

07569665

ABSTRACT:
The present invention relates generally to a molecular marker of the integrity of the extracellular matrix in an animal including a human subject. More particularly, the present invention provides a molecular marker of cartilage integrity. The identification of the molecular marker in circulatory or tissue fluid is indicative of disrepair of the extracellular matrix and in particular cartilage such as caused or facilitated by trauma or a degenerative disease or other condition, for example, arthritis or autoimmunity. The molecular marker is preferably in the form of a glycoprotein but the instant invention extends to genetic sequences encoding the polypeptide portion of the glycoprotein. Expression analysis of such genetic sequences provides predictive utility in detecting normal or abnormal extracellular matrix development. The identification of the molecular marker of the present invention enables the development of a range of diagnostic and therapeutic agents for degeneration of extracellular matrix or the poor development of the matrix at the fetal and postnatal stages including testing for mutations in the gene sequence in human disease such as but not limited to cartilage disease or arthritis. In a most preferred embodiment, the molecular marker is referred to herein as “WARP” for von Willebrand Factor A-Related Protein. The corresponding genetic form of WARP is referred to herein as “WARP”.

REFERENCES:
patent: 4016043 (1977-04-01), Schuurs et al.
patent: 4018653 (1977-04-01), Mennen
patent: 4424279 (1984-01-01), Bohn et al.
patent: 7129338 (2006-10-01), Ota et al.
patent: 7368531 (2008-05-01), Rosen et al.
patent: 2006/0003323 (2006-01-01), Alsobrook et al.
patent: 1067182 (2001-01-01), None
patent: 9853071 (1998-11-01), None
patent: 0058473 (2000-10-01), None
patent: 0118022 (2001-03-01), None
patent: 0142285 (2001-06-01), None
Attwood TK. Genomics. The Babel of bioinformatics. Science. 290(5491):471-473, 2000.
Skolnick et al. From genes to protein structure and function: novel applications of computational approaches in the genomic era. Trends Biotechnol. 18(1):34-9, 2000.
Bork Powers and pitfalls in sequence analysis: the 70% hurdle. Genome Res. Apr. 2000;10(4):398-400.
Doerks et al. Protein annotation: detective work for function prediction. Trends Genet. Jun. 1998;14(6):248-50.
Arikawa-Hirasawa et al., Dyssegmental dysplasia, Silverman-Handmaker type, is caused by functional null mutations of the perlecan gene.Nat. Genet. Apr. 2001;27(4):431-4.
Arikawa-Hirasawa et al., “Perlecan is essential for cartilage and cephalic development,” Nat Genet. Nov. 1999;23(3):354-8.
GenPept Database Acc. No. AAH 26919, submitted Apr. 4, 2002.
Fitzgerald et al., (2001) “A new FACIT of the collagen family: COL21A1”, FEBS Letters 505:275-280.
Koch et al. (2001) “α1(XX) collagen, a new member of the collagen subfamily, fibril-associated collagens with interrupted triple helices”, J. Biol. Chem. 276: 23120-23126.
Dgene Database Acc. No. AAB 88340, entered May 23, 2001.
GenPept Database Acc. No. AAK 38350, submitted Apr. 11, 2001.
GenPept Database Acc. No. AAB 42581, entered Feb. 8, 2001.
Fitzgerald et al. (2001) “The N-terminal N5 subdomain of the α3(VI) chain is important for collagen VI microfibril formation”, J. Biol. Chem. 276: 187-193.
Gilges et al. (2000) “Polydom: a secreted protein with pentraxin, complement control protein, epidermal growth factor and von Willebrand factor A domains”, Biochem J. 352: 49-59.
Chen et al. (1999) “Assembly of a novel cartilage matrix protein filamentous network: molecular basis of a differential requirement of von Willebrand factor a domains”, Mol. Biol. Cell 10: 2149-2162.
Deák et al. (1999) “The matrilins: a novel family of oligomeric extracellular matrix proteins”, Matrix Biology 18: 55-64.
GenPept Database Acc. No. AI 115125, entered Sep. 2, 1998.
GenBank Acc. No. NP 038620, published in 1998.
Emsley et al. (1998) “Crystal structure of the von Willebrand factor A1 domain and implications for the binding of platelet glycoprotein Ib”, J. Biol. Chem. 273: 10396-10401.
Emsley et al. (1997) “Crystal structure of the I domain from integrin α2β1”, J. Biol. Chem. 272: 28512-28517.
Kuo et al. (1997) “Type VI collagen anchors endothelial basement membranes by interacting with type IV collagen”, J. Biol. Chem. 272: 26522-26529.
Beinkowska et al. (1997) “The von Willebrand factor A3 domain does not contain a metal ion-dependent adhesion site motif”, J. Biol. Chem. 272: 25162-25167.
Zaverio M. Ruggeri (1997) “Perspectives series: cell adhesion in vascular biology”, J. Clin. Invest., 99: 559-564.
Robertson et al., (1997) “Mapping and characterization of a novel cochlear gene in human and in mouse: a positional candidate gene for a deafness disorder, DFNA9”, Genomics 46: 345-354.
GenBank Acc. No. O 42163, submitted in Jul. 1997.
GenBank Acc. No. NP 058042, published in 1997.
GenBank Acc. No. NP 034900, published in 1997.
Beck et al. (1996) “The C-terminal domain of cartilage matrix protein assembles into a triple-stranded α-helical coiled-coil structure”, J. Mol. Biol. 256: 909-923.
Chan et al. (1996) “Site-directed mutagenesis of human type X collagen”, J. Biol. Chem. 271: 13566-13572.
Tuckwell et al. (1996) “The A-domain of integrin α2 binds specifically to a range of collagens but is not a general receptor for the collagenous motif”, Eur. J. Biochem. 241: 732-739.
GenBank Acc. No. NP 034899, published in 1996.
GenBank Acc. No. 1589549, published in 1996.
GenBank Acc. No. P11276, submitted Dec. 1995.
Qu et al. (1995) “Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, αLβ2) integrin”, Proc. Natl. Acad. Sci. USA 92: 10277-10281.
Haudenschild et al. (1995) “The role of coiled-coil α-helices and disulfide bonds in the assembly and stabilization of cartilage matrix protein subunits”, J. Biol. Chem. 270: 23150-23154.
Lee et al. (1995) “Crystal structure of the domain from the α subunit of integrin CR3 (CDb/CD18)”, Cell 80: 631-638.
Hansen et al. (1995) “Prediction of O-glycosylation of mammalian proteins: specificity patterns of UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase”, Biochem. J. 308: 801-813.
Chan et al. (1995) “The three heavy-chain precursors for the inter-α-inhibitor family in mouse: new members of the multicopper oxidase protein group with differential transcription in liver and brain”, Biochem. J. 306: 505-512.
Lefebvre et al. (1995) “Type X collagen gene expression in mouse chondrocytes immortalized by a temperature-sensitive simian virus 40 large tumor antigen”, J. Cell Biol. 128: 239-245.
Engel et al. (1994) “Domain organizations of extracellular matrix proteins and their evolution”, Development Supplement: 35-42.
Thompson et al. (1994) “Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice”, Nucleic Acids Res. 22: 4673-4680.
McMahon et al. (1994) “C2C12cells: biophysical, biochemical, and immunocytochemical properties”, Am. J. Physiol. 266: 1795-1802.
Denis et al. (1993) “Localization of von Willebrand factor binding domains to endothelial extracellular matrix and to type VI collagen”, Arterioscler. Thromb. 13: 398-406.
Chan et al. (1993) “Characterization of an arginine 789 to cysteine substitution in α1(II) collagen chains of a patient with spondyloepiphyseal dysplasia”, J. Biol. Chem. 268: 15238-15245.
Colombatti et al. (1993) “Type A modules: interacting domains found in several non-fibrillar collagens and in other extracellular matrix proteins”, Matrix 13: 297-306.
GenBank Acc. No. P 56199, published in 1993.
GenBank Acc

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