Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-26
1998-01-13
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435 915, 435 5, 536 243, 536 2433, C12Q 168, C12Q 170, C12P 1934, C07N 2104
Patent
active
057078076
ABSTRACT:
This invention relates to a method for classifying (indexing) cDNA which has been reverse-transcribed from tissue- or cell-derived RNA, or DNA in a short period without duplication by using class-IIS restriction enzymes or a combination of a class-IIS and a class-II restriction enzymes. According to this invention, it is possible to analyse and diagnose variations such as tumors easily, correctly and promptly by comparing the analyzed pattern of genes expressed in a cell or tissue sample with the analyzed pattern of normal genes. This method is also applicable to the search and isolation of genes of physiologically active substances that are potential pharmaceuticals or causative genes of hereditary diseases, as well as the isolation of those genes that are useful for improving agricultural products.
REFERENCES:
patent: 5508169 (1996-04-01), Deugau
K. Kato, 1995, "Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes", Nuc. Acids Res. 23:3685-3690.
McClelland et al., 1995, "RNA fingerprinting and differential display using arbitrarily primed PCR", TIG 11:242-246.
Schena et al., 1995, "Quantitative monitoring of gene expression patterns with a complementary DNA microarray", Science 270:467-470.
D.R. Smith, 1992, "Ligation-mediated PCR of restriction fragments from large DNA molecules", PCR Methods and Applications 2:21-27.
Velculescu et al., 1995, "Serial analysis of gene expression", Science 270:484-487.
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Hakvoort et al. Nucleic Acids Research 22:879-79, 1994.
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Unrau et al. Gene 145: 163-169, 1994.
Jones W. Gary
Rees Dianne
Research Development Corporation of Japan
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