Molecular identification of Aspergillus species

Details Molecular identification of Aspergillus species Molecular identification of Aspergillus species

2011-01-18

2011-01-18

Helms, Larry R (Department: 1645)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S023700, C536S024320

Reexamination Certificate

active

07871779

ABSTRACT:
Novel techniques for the detection ofAspergillusin samples are disclosed. These techniques relate to PCR amplification and/or detection ofAspergillusITS1 rDNA sequences, and the identification of particular species ofAspergillusby detecting differences in the ITS1-V1, ITS-V2, ITS-V3, ITS-V4, and ITS-V5 nucleic acid sequences ofAspergillus. The highly variable regions of the ITS1 rDNA sequences are particularly useful in distinguishing, for example,Aspergillus clavatus, Aspergillus granulosus, Aspergillus sydowii, Aspergillus flavipes, Aspergillus restrictus, Aspergillus versicolor, Aspergillus wentii, andAspergillus chevalieri. In particular embodiments, the sequence differences are also able to distinguish among variants of particular species, such asAspergillus granulosusCBS 119.5A,Aspergillus granulosusstrain NRRL 1932,Aspergillus sydowiistrain NRRL 250,Aspergillus sydowiistrain NRRL 4768,Aspergillus sydowiistrain CUHI,Aspergillus sydowiistrain CUH2,Aspergillus sydowiistrain CUH7, andAspergillus sydowiistrain CUH8.

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