Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-12-19
1998-11-24
Elliott, George C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 4351721, 435 691, 435243, 4352521, 4352523, 4353201, 536 231, C12N 100, C12N 121, C12N 1500, C12Q 168
Patent
active
058405569
ABSTRACT:
Tools for genetically engineering Pasteurellaceae are provided. Replication-conditional plasmids which are useful for the Pasteurellaceae have been isolated and characterized. The plasmids can be utilized for delivery of DNA segments into the Pasteurellaceae in situations where control of extrachromosomal replication desired, such as in achieving allelic exchange or site-directed mutagenesis.
A restriction endonuclease, HsoI, was isolated from a bovine lung isolate of Haemophilus somnus. The enzyme was found to be a true isoschizomer of HinPI, a commercially available enzyme originally isolated from Haemophilus influenzae PI. Commercially available HhaI methyl transferase was found to protect against cleavage by both enzymes. Methylation of foreign plasmid DNA was found to enhance transformation of Haemophilus somnus in excess of four orders of magnitude.
REFERENCES:
patent: 5587305 (1996-12-01), Briggs et al.
Briggs et al., "Isolation of a Cryptic Plasmic from Pasteurella haemolytica by Electroporation," Abstract, 72nd Annual Meeting of the Conference of Research Workers in Animal Disease, Nov. 11, 1991.
Craig et al., "A Plasmid Which Can Be Transferred Between Eschirichia coil and Pasteurella haemolytica by Electroporation and Conjugation," J. Gen. Microbiology, 135:2885-2890 (1989).
Homchampa et al., "Construction and Vaccine Potential of an AroA Mutant of Pasteurella haemolytica", Veterinary Microbiology 42:35-44 (1994).
Briggs et al., "Characterizatin of a Restriction Endonuclease, PhaI, from Pasteurella haemolytica Serotype A1 and Protection of Heterologous DNA by a Cloned PhaI Methyltransferase Gene", Applied and Environmental Microbiology 60(6):2006-2010 (1994).
Tatum et al., "Molecular Gene Cloning and Nucleotide Sequencing and Construction of an aroA Mutant of Pasteurella haemolytica Serotype A1", Applied and Environmental Microbiology 60(6):2011-2016 (1994).
Briggs Robert E.
Tatum Fred M.
Biotechnology Research And Development Corporation
Elliott George C.
McGarry Sean
The United States of America as represented by the Department of
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