Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...
Reexamination Certificate
2007-12-11
2007-12-11
Gakh, Yelena G. (Department: 1743)
Chemistry: analytical and immunological testing
Nuclear magnetic resonance, electron spin resonance or other...
C436S071000
Reexamination Certificate
active
10606601
ABSTRACT:
A method for determination of at least one of the triglyceride molecular species in a biological sample comprising the subjecting sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to 2D electrospray ionization tandem mass spectrometry (ESI/MS/MS) with neutral loss scanning of all naturally occurring aliphatic chains and contour analysis of 2D intercept peaks. A method for determination of tricylglyceride content and/or TG molecular species directly from a lipid extract of a biological sample comprising subjecting said lipid extract to electrospray ionization tandem mass spectrometry.
REFERENCES:
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Brügger et al. “Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry”,Proc. Natl. Acad. Sci.,USA, Mar. 1997, vo. 94, pp. 2339-2344.
Koivusalo et al. “Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response”, J. Lipid. Res., Apr. 2001, v. 42, pp. 663-672.
Han, X. and Gross, R.W. “Quantitative Analysis and Molecular Species Fingerprinting of Triacylglyceride Molecular Species Directly from Lipid Extracts of Biological Sample by Electrospray Ionization Tandem Mass Spectrometry”, Anal. Biochem., 2001, v. 295, pp. 88-100 (published on-line Jun. 27, 2001).
Houjou et al. “Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3”, Rapid Comm. Mass Spectrom., 2004, v. 18, pp. 3123-3130.
Gross Richard W.
Han Xianlin
Armstrong Teasdale LLP
Gakh Yelena G.
Washington University in St. Louis
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