Molecular diversity screening method

Details Molecular diversity screening method Molecular diversity screening method

1996-05-10

1999-02-16

Housel, James C.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

21032163, 422 681, 435 71, 435 78, 4352872, 436536, 436824, 530414, G01N 33537, B01D 6300

Patent

active

058720154

ABSTRACT:
A device and method is disclosed for the identification, concentration and isolation of a chemical compound from a mixture of compounds on the basis of the binding affinity of the chemical compound to a macromolecule or complex thereof, that is, to a receptor. Both the compounds to be assayed, and the macromolecules to which the compounds may bind, are free in solution. The method comprises contacting a mixture of compounds in solution with a solution of macromolecules or complexes thereof in a device possessing an ultrafiltration membrane to retain the receptor and bound compound receptor complex; washing through the device non-bound compounds; and releasing a bolus of the bound compound which passes through the ultrafiltration membrane where it may be analyzed chemically and/or spectrally (e.g. mass spectrometry) and/or bioassayed. The device includes a barrier such as an ultrafiltration membrane separating a binding chamber and an effluent chamber. An inlet port and wash port connect to the binding chamber. An effluent port connected to the effluent chamber is directed to analyzer equipment. Stirring means may be located within the binding chamber.

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