Molecular detection and quantification of Enterococci

Details Molecular detection and quantification of Enterococci Molecular detection and quantification of Enterococci

2011-05-24

2011-05-24

Zeman, Robert A (Department: 1645)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024330

Reexamination Certificate

active

07947441

ABSTRACT:
A primer pair and probe for the large subunit ribosomal RNA gene of enterococci for use in a real-time nucleic acid sequence based amplification (NASBA) assay.

REFERENCES:
patent: 4851330 (1989-07-01), Kohne
patent: 5334501 (1994-08-01), Adams et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5650290 (1997-07-01), Grant
patent: 5695946 (1997-12-01), Benjamin et al.
patent: 5723597 (1998-03-01), Kohne
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6355421 (2002-03-01), Coull et al.
patent: 6583275 (2003-06-01), Doucette-Stamm et al.
patent: 6821770 (2004-11-01), Hogan
patent: 7118870 (2006-10-01), Field et al.
patent: 7205112 (2007-04-01), Paul et al.
patent: 7422857 (2008-09-01), Paul
patent: 7563577 (2009-07-01), Paul et al.
patent: 7700278 (2010-04-01), Paul et al.
patent: 7888031 (2011-02-01), Paul
Bachoon, D.; Gates, K.; King, E. 2005. Rapid Detection of human fecal contamination in estuarine environments by PCR targeting of Bifidobacterium adolescentis. Journal of Microbiological Methods. 68: 76-81.
Baeummer, A.J.; Leonard, B.; McElwee, J.; Montangna, R.A. 2004. A rapid biosensor for viable B. anthracic spores. Anal. Bioanal. Chem. 380: 15-23.
Buckalew, D.W.; Hartman, L.J.; Grimsley, G.A.; Martin, A.E.; Register, K.M. 2006. A long-term study comparing membrane filtration with Colilert defined substrates in detecting fecal coliforms andEscherichia coliin natural waters. Journal of Environmental Management. 80: 191-197.
Fiksdal, L.; Pommepuy, M.; Caprais, M.; Midttun, I. 1994. Monitoring of Fecal Pollution in Coastal Waters by Use of Rapid Enzymatic Techniques. Applied and Environmental Microbiology. 60: 1581-1584.
He, J.; Jiang, S. 2005. Quantification of Enterococci and Human Adenoviruses in Environmental Samples by Real-Time PCR. Applied and Environmental Microbiology. 71: 2250-2255.
Loens, K.; Beck, T.; Goossens, H.; Ursi, D.; Overdijk, M.; Sillekens, P.; Ieven, M. 2006. Development of conventional and real-time NASBA for the detection of Legionella species in respiratory specimens. Journal of Microbiological Methods. 67: 408-415.
Noble, R.T.; Weisberg, S.B.; Leecaster, M.K.; McGee, C.D.; Ritter, K.; Walker, K.O.; Vainik, P.M. 2003. Comparison of Beach Bacterial Water Quality Indicator Measurement Methods. Environmental Monitoring and Assessment. 81: 301-312.
Omar, N. B.; Castro, A.; Abriouel, H.; Lucas, R.; Perez, R.; Martinez-Canamero, M.; Galvez, A. 2004. Quantification ofEnterococcus faecalisandEnterococcus faeciumin different foods using rRNA-targeted oligonucleotide probes. Journal of Microbiological Methods. 61: 187-192.
Vail, J.H.; Morgan, R.; Merino, C.R.; Gonzales, F.; Miller, R.; Ram, J.L. 2003. Enumeration of WaterborneEscherichia coliwith Petrifilm Plates: Comparison and Standard Methods. J. Environ. Qual. 32: 368-373.
Van Der Wolf, J.M.; Van Beckhoven, J.R.C.M.; De Haan, E.G.; Van Den Bovenkamp, G.W.; Leone, G.O. M. 2004. Specific detection ofRalstonia solanacearum16S rRNA sequences by AmpliDet RNA. European Journal of Plant Pathology. 110: 25-33.
Haugland, R.A.; Siefring, S.C.; Wymer, L.J.; Brenner, K.P.; Dufour, A.P. 2005. Comparison ofEnterococcusmeasurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis. Water Research. 39: 559-568.
Frahm, E.; Obst, U. 2003. Application of the fluorogenic probe technique (TaqMan PCR) to the detection ofEnterococcusspp. andEscherichia coliin water samples. Journal of Microbiological Methods. 52: 123-131.
Behlke et al. 2005. “Fluorescence and Fluorescence Applications.” Integrated DNA Technologies. pp. 1-13.
Weusten et al. 2002. “Principles of Quantitation of Viral Loads Using Nucleic Acid Sequence-Based Amplification in Combination with Homogenous Detection Using Molecular Beacons.” Nucleic Acids Research. vol. 30. No. 6 e26. pp. 1-7.
Casper, E.T. et al. “A handheld NASBA analyzer for the field detection and quantification ofKarena brevis” Harmful Algae, Jan. 2007, 6(1):112-118.
Casper, E.T. et al. “Detection and Quantification of the Red Tide DinoflagellateKarena brevisby Real-Time Nucleic Acid Sequence-Based Amplification”Applied and Environmental Microbiology, Aug. 2004, 70(8):4727-4732.
Patterson, S.S. et al. “A nucleic acid sequence-based amplification assay for real-time detection of norovirus genogroup II”Journal of Applied Microbiology, Oct. 2006, 101(4):956-963.
Patterson, S.S. et al. “Increased precision of microbial RNA quantification using NASBA with an internal control”Journal of Microbiological Methods, 2005, 60:343-352.
International Search Report dated Nov. 13, 2007 for International Application No. PCT/US2007/009235, 2 pages.
International Preliminary Report on Patentability dated Oct. 14, 2008 for International Application No. PCT/US2007/009235, 4 pages.

Affiliated with

Also associated with

Rating

Molecular detection and quantification of Enterococci does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Molecular detection and quantification of Enterococci, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecular detection and quantification of Enterococci will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2679809