Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1983-03-02
1986-06-17
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435253, 435317, 435839, 935 27, 935 56, 935 74, C12R 1125, C12N 1500, C12N 120, C12N 100, C12P 2100
Patent
active
045956600
ABSTRACT:
A mutant of Bacillus subtilis has been isolated which greatly facilitates gene cloning in this nonpathogenic microorganism. B. subtilis is a known protein secretor and can be used efficiently in commercial operations. Unlike the more commonly used clone-propagating organism E. coli., B. subtilis has the advantage of lacking pyrogenic substances in its cell envelope. However, chimeric plasmids for infection of B. subtilis have been difficult to prepare, and if E. Coli is used as an intermediate host to provide plasmid forms suitable for Bacillus transformation, the B. subtilis treats any E. coli-propagated DNA as foreign and preferentially attacks the insert portion of the plasmid. This attach results in loss of cloned genes and limits the use of B. subtilis as a cloning system. The B. subtilis recipient strain of this invention is, on the other hand, stably and efficiently transformed by E. coli-propagated plasmid DNA at high frequency. The discovery of this mutant and the substantially higher frequency stable transformants obtained from it greatly facilitates the use of B. subtilis as a host for the expression of cloned genes.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4302544 (1981-11-01), Young et al.
patent: 4419450 (1983-12-01), Dean et al.
Tanaka, T., Mol Gren Genet, vol. 175, p. 235, 1979.
Gryczan et al, J of Bact., v. 134, No. 1, Apr. 1978, "Characterization of Staphylococcus aureus Plasmids Introduced by Transformation into Bacillus subtilis".
Gryczan et al, Proc Nat'l Acad Sci, v. 75, No. 3, pp. 1428-1432, Mar. '78, "Construction and Properties of Chimeric Plasmids in Bacillus subtilis".
Lovett et al., Chemical Abstracts, v. 94, No. 80124x, 1981, "Molecular Cloning of Bacillus subtilis Using Plasmid Vectors".
Gray et al, J of Bact., v. 145, No. 1, pp. 422-428, Jan. 1981, "Molecular Cloning and Expression of Bacillus licheniformis .beta. lactamase Gene in Escherichia coli and Bacillus subtilis".
Keggins et al, Proc. Nat'l Acad Sci, v 75, No. 3, pp. 1423-1427, Mar. 1978, "Molecular Cloning of Genetically Active Fragments of Bacillus DNA in Bacillus subtilis and Properties of the Vector Plasmid pUB110".
Ehrlichs, Proc. Nat'l Acad Sci, v 75, No. 3, pp. 1433-1436, Mar. '78, "DNA Cloning in Bacillus subtilis".
Gryczan et al, J of Bact., v. 141, No. 1, Jan. 1980, "Characterization of Chimeric Plasmid Cloning Vehicles in Bacillus subtilis".
Ostroff Gary R.
Pene Jacques J.
Teskin Robin L.
University of Delaware
Wiseman Thomas G.
LandOfFree
Molecular cloning with bifunctional plasmid vectors in Bacillus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Molecular cloning with bifunctional plasmid vectors in Bacillus , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecular cloning with bifunctional plasmid vectors in Bacillus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2273229