Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1983-01-31
1985-04-02
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 68, 435 91, 435317, 4351723, 435875, 435 70, 935 29, 935 56, 935 60, 935 72, C12N 120, C12N 100, C12N 1500, C12P 2100, C12P 1934, C12P 2102, C12R 1385
Patent
active
045088276
ABSTRACT:
Improved cloning vectors derived from pRO1614 are described. One of these vectors, pRO1727, is suitable for cloning using DNA cleaved with the restriction endonuclease, PstI, and allows selection for the recovery of recombinant plasmids using tetracycline resistance. The cloning efficiency observed for pRO1727 is higher than described previously for pRO1614 and the host range of this vector is now restricted to Pseudomonas bacteria. Another vector, designated pRO1729, is described and developed from pRO1727 by deletion of a portion of its DNA and incorporation of a segment of DNA which encodes for resistance to the antibiotic, chloramphenicol. The chloramphenicol resistance determinant has a cleavage site for restriction endonuclease EcoRI within its chloramphenicol resistance determinant. Thus, DNA cloned into this site results in the loss of chloramphenicol resistance which can be detected subsequent to a cloning experiment. Both pRO1727 and pRO1729 are more useful in Pseudomonas for cloning than pRO1614.
REFERENCES:
patent: 3813316 (1974-05-01), Chakrabarty
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4278765 (1981-07-01), Debabov et al.
patent: 4374200 (1983-02-01), Olsen
Bolivar, R., Gene 4:121-136 (1978).
Royle, P. Matsumoto, and B. Holloway, J. Bacteriol. 145:145-155 (1981).
Bolivar, R. R. Rodriguez, P. Greene, M. Betlach, H. Heyneker, H. Boyer, J. Cross, and B. Falkow, Gene 2:95-113 (1977).
Olsen, R. and P. Shipley, J. Bacteriol. 113:772-780 (1972).
Stanier, R., N. Palleroni, and M. Doudoroff, J. Gen. Microbiol. 43:159-271 (1966).
Guerry, LeBlanc and Falkow, J. Bacteriol. 116:1064-116:1066 (1973).
Hansen and Olsen, J. Bacteriol. 135:227-238 (1978).
Mercer and Loutit, J. Bacteriol. 140:37-42 (1979).
Davis, Botstein and Roth, In Advanced Bacteriol Genetics: A Manual for Genetic Engineering, Cold Spring Harbor Lab., (1980 pp. 134-137).
Martinell James
McLeod Ian C.
Microlife Technics, Inc.
Shapiro Lionel M.
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