Molecular cloning of RNA using RNA ligase and synthetic oligonuc

Details Molecular cloning of RNA using RNA ligase and synthetic oligonuc Molecular cloning of RNA using RNA ligase and synthetic oligonuc

1983-05-03

1987-04-28

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 91, 435253, 435317, 536 27, C12P 2100, C12P 1934, C12N 120, C12N 100, C07H 1512

Patent

active

046614508

ABSTRACT:
Methods and compositions for the insertion and molecular cloning of RNA in DNA cloning vectors are described. RNA molecules to be cloned are modified by the ligation of oligonucleotide linkers onto the termini of the RNA molecule using T4 RNA ligase. Such linkers may be composed of DNA, RNA, or mixtures of each and facilitate the insertion and ligation of the RNA species into a DNA cloning vector. The resulting recombinant vectors are used to transform host cells which provide for the generation of multiple DNA copies of the RNA molecule.

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