Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-02-29
1993-06-08
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 435221, 43525231, 4353201, C12P 2400, C12N 1500, B12N 1575
Patent
active
052178781
ABSTRACT:
Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.
REFERENCES:
patent: 4002572 (1977-01-01), te Nijenhuis
Lewin; Science 237: 1570 (1987).
Reeck et al.; Cell 50: 667 (1987).
Shen et al.; Cell 26: 191 (1981).
Wells et al; Nucleic Acids Res. 11: 7911 (1983).
Stahl et al; J. Bacteriol. 158: 411 (1984).
Cornfield et al., "A modified protoplast-regeneration protocol facilitating the detection of cloned exoenzyme genes in Bacillus subtilis" Gene (1984) 30:17-22.
J. H. Yu et al., "Physiological properties and transformation of alkaline-tolerant bacteria" Biological Abstracts (1986) 82:871 Abstr. No. 86427.
Usami et al., "Plasmids in Alkalophilic Bacillus sp." Agricultural and Biological Chemistry (1983) 47:2101-2102.
Wong et al., "The subtilisin E gene of Bacillus subtilis is transcribed from a .sigma..sup.37 promoter in vitro" Proc. Natl. Acad. Sci. USA (1984) 81:1184-1188.
Thomas et al., "Tailoring the pH dependence of enzyme catalysis using protein engineering" Nature (1985) 318:375-376.
Pak et al., "Cloning of protease gene produced by heat-resistant alkalinic Bacillus in Escherichia coli" Biological Abstracts/RRM, Ref No. 34060253 title only.
Horikoshi et al., "Genetic Applications of Alkalophilic Microorganisms" Spec. Publ. Soc. Gen. Microbiol. (1986) 17:297-315.
Vasantha et al., "Cloning of a Serine Protease Gene from Bacillus amyloliquefaciens and Its Expression in Bacillus subtilis" Genetics and Biotechnology of Bacilli (Proc. Int. Conf. 2nd International Conf. (Jul. 1983) pp. 163-172.
Hofemeister et al., "Integration of plasmid pE194 at multiple sites on the Bacillus subtilis chromosomes" Chemical Abstracts (1983) 98:88.
Prozorov et al., "Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector" Gene (1985) 34:39-46.
Mulleners Leo J. S. M.
van der Laan Johannes C.
van Eekelen Christiaan A. G.
Gist-brocades, NV
Martinell James
Rae-Venter Barbara
Rowland Bertram I.
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