Molecular cloning and expression of a vibrio proteolyticus neutr

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435212, 435220, 43525233, 435320, 435 712, 435 91, 4351721, 530 27, 935 19, 935 29, 935 41, 935 56, 935 73, 935 82, C12N 948, C12N 952, C12N 120, C12N 100, C12N 1500, C12P 2104, C12P 1934, C07H 1512, C07K 1300

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049668463

ABSTRACT:
Neutral protease genes from Vibrio proteolyticus or Bacillus can be cloned and expressed in gram-negative microorganisms, such as E. coli or Serratia. The functional neutral protease enzyme is expressed.

REFERENCES:
patent: 3796635 (1974-03-01), Delente et al.
Nakahama, K. et al Nucl. Acids Res. vol. 14, pp. 5843-5844 (1986).
Maniatis, T. et al, Molecular Cloning, A laboratory Manual, Cold Spring Harbor Laboratory CHS, NY (1982).
Wells, J. A. et al, Nucl. Acids Res. vol. 11, pp. 7911-7925 (1983).
Yang, M. Y. et al, J. Baet. vol. 160, pp. 15-21 (1984).
Kaihat, Heat Stable Protease Production Japanese Patent Publication, 1983.
Takaji, M. et al, J. Bact. vol. 163, pp. 824-831 (1985).

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