Molecular cloning and characterization of the feline immunodefic

Details Molecular cloning and characterization of the feline immunodefic Molecular cloning and characterization of the feline immunodefic

1994-10-18

1998-04-07

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435 9133, 4352523, 4241871, 4242071, 536 2372, C12N 700, C12N 701, C12N 120, C12P 1934

Patent

active

057363781

ABSTRACT:
Molecular clones of the feline immunodeficiency virus isolate PPR (FIV.sub.PPR) were obtained from a genomic library prepared from infected feline peripheral blood lymphocytes (PBLs). FIV.sub.PPR infected and replicated efficiently in feline PBLs but not Crandall feline kidney (CRFK) or G355-5 cells. In contrast, a clone designated 34TF10 of the prototypical FIV Petaluma isolate (FIV.sub.Pet) replicated inefficiently on feline PBLs while readily infecting and replicating in CRFK and G355-5 cells. The 34TF10 and PPR clones have an overall nucleic acid sequence identity of 91% while the env genes display only 85% conservation at the amino acid level. The long terminal repeats (LTRs) were 7% divergent between the two clones, with a lack of conservation in putative NF-.kappa.B, LBP-1, and CCAAT enhancer promoter sites. Full-length proviral clones will provide important biochemic, immunologic, and diagnostic reagents.

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