Molecular clone of a chitinase gene from vibrio parahemolyticus

Details Molecular clone of a chitinase gene from vibrio parahemolyticus Molecular clone of a chitinase gene from vibrio parahemolyticus

1992-04-29

1994-10-04

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435220, 4353201, 435909, 536 232, 536 237, C12N 121, C12N 1531, C12N 1552, C12N 1570

Patent

active

053526074

ABSTRACT:
A process for cloning the chitinase gene of Vibrio parahemolyticus is provided, comprising the steps of cleaving the Vibrio parahemolyticus DNA with Sau3A, PSTI or other restriction enzyme, mixing the cleaved DNA fragments in the presence of pUC18 and T4 ligase to produce a composite plasmid, and inserting the composite plasmid in a DH5a strain of E. Coli.

REFERENCES:
patent: 4751081 (1988-06-01), Suslow et al.
Soto-Gil et al., 1985, Chemical Abstracts, vol. 102, Abstract No. 21624g, p. 180.
Ohtakara et al., 1979, Chemical Abstracts, vol. 91, Abstract No. 86189g, p. 320.

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