Modulators of ribosomal function and identification thereof

Details Modulators of ribosomal function and identification thereof Modulators of ribosomal function and identification thereof

2005-10-04

2005-10-04

Allen, Marianne P. (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C702S020000, C702S027000, C435S006120

Reexamination Certificate

active

06952650

ABSTRACT:
The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

REFERENCES:
patent: 5180719 (1993-01-01), White et al.
patent: 5281703 (1994-01-01), White et al.
patent: 5336768 (1994-08-01), Albrecht et al.
patent: 5466681 (1995-11-01), Krivan et al.
patent: 5693791 (1997-12-01), Truett
patent: 5866549 (1999-02-01), Or et al.
patent: 5905144 (1999-05-01), Truett
patent: 6380356 (2002-04-01), Griffin et al.
patent: 6437119 (2002-08-01), Truett
patent: 6446032 (2002-09-01), Schimmel
patent: 6468979 (2002-10-01), Pellacini et al.
patent: 6638908 (2003-10-01), Steitz et al.
patent: 2002/0022257 (2002-02-01), Suh et al.
patent: 2002/0086308 (2002-07-01), Steitz et al.
patent: 2002/0188108 (2002-12-01), Noller et al.
patent: 2003/0027315 (2003-02-01), Yonath et al.
patent: 2003/0171327 (2003-09-01), Steitz et al.
patent: 2003/0232779 (2003-12-01), Steitz et al.
patent: 1 172 374 (2002-01-01), None
patent: 1 186 614 (2002-03-01), None
patent: 1 188 769 (2002-03-01), None
patent: WO 95/07271 (1995-03-01), None
patent: WO 96/18633 (1996-06-01), None
patent: WO 97/35195 (1997-09-01), None
patent: WO 99/63937 (1999-12-01), None
patent: WO 00/32619 (2000-06-01), None
patent: WO 01/80863 (2001-11-01), None
Hansen et al., “Structural Insights Into Peptide Bond Formation,”Proc. Natl. Acad. Sci. USA, 99(18):11670-11675 (2002).
Hansen et al., “The Stuctures of Four Macrolide Antibiotics Bound to the Large Ribosomal Subunit,”Molecular Cell, 10:117-128 (2002).
Klein et al., “The Kink-Turn: A New RNA Secondary Structure Motif,”EMBO J., 20(15):4214-4221 (2001).
Nissen et al., “RNA Tertiary Interactions in the Large Ribosomal Subunit: The A-Minor Motif,”Proc. Natl. Acad. Sci. USA, 98(9):4899-4903 (2001).
Schmeing et al., “A Pre-Translocational Intermediate in Protein Synthesis Observed in Crystals of Enzymatically Active 50S Subunits,”Nature Struct. Biol., 9(3):225-230 (2002).
Drenth, “Principles of Protein X-ray Crystallography,” 1994. Springer-Verlag, pp. 1-18.
Franceshi et al. (1993) “Towards Atomic Resolution of Prokaryotic Ribosomes: Crystallographic, Genetic and Biochemical Studies” inThe Translational Apparatus397-410.
Hope et al. (1989) “Cryocrystallography of Ribosomal Particles”Acta. Cryst. B45: 190-199.
Shevack et al. (1985) “Characterization and Crystallization of Ribosomal Particles fromHalobacterium Marismortui” FEBS Letters184(1):68-71.
Yonath et al. (1980) “Crystallization of the Large Ribosomal Subunits fromBacillus Stearothermophilus” Biochem. Intern'l. 1(5):428-435.
Yonath et al. (1984) “Some X-ray Diffraction Patterns from Single Crystals of the Large Ribosomal Subunit fromBacillus Stearothermophilus” J. Mol. Biol. 177:201-206.
Yonath et al.(1988) “Crystallography of Ribosomal Particles”J. Cryst. Growth90:231-244.
Hansen, et al. (2003) “Structures of Five Antibiotics Bound at the Peptidyl Transferase Center of the Large Ribosomal Subunit,”J. Mol. Biol. 330:1061-1075.
Fourmy et al. (1996) “Structure of the A Site ofEscherichia coli16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic”Science274(5291):1367-1371.
Hermann et al. (1999) “Docking of Cationic Antibiotics to Negatively Charged Pockets in RNA Folds”J. Med. Chem. 42(7):1250-1261.
Ioannou et al. (1998) “Kinetics of Inhibition of Rabbit Reticulocyte Peptidyltransferase by Anisomycin and Sparsomycin”Molecular Pharmacology53(6):1089-1096.
Kirillov et al. (1999) “Peptidyl Transferase Antibiotics Perturb the Relative Positioning of the 3'-Terminal Adenosine of P/P'-Site-Bound tRNA and 23S rRNA in the Ribosome”RNA5(8):1003-1013.
Spickler et al. (1997) “Streptomycin Binds to the Decoding Center of 16 S Ribosomal RNA”J. Mol. Biol. 273(3):586-599.
Wong et al. (1998) “Specificity of Aminoglycoside Antibiotics for the A-Site of the Decoding Region of Ribosomal RNA”Chemistry&Biology5(7):397-406.
European Search Report for Application No. 02255442.2 dated Mar. 6, 2003.
Hanessian et al. (1984) ““Quantamycin”: A Computer-Simulated New-Generation Inhibitor of Bacterial Ribosomal Binding”Journal American Chemical Society106:6114-6115.
Vince et al. (1975) “Chloramphenicol Binding Site with Analogues of Chloramphenicol and Puromycin”Antimicrobial Agents and Chemotherapy8(4):439-443.
Shuker et al. (1996) “Discovering High-Affinity Ligands for Proteins: SAR by NMR”Science274(5292):1531-1541.
Hecker et al. (1993) “Application of Hygromycin A Structure Activity Relationships to the Antibiotic A201A”Bioorganic&Medicinal Chemistry Letters3(2):295-298.
Wang et al. (1997) “Dimeric Aminoglycosides: Design, Synthesis and RNA Binding”Bioorganic&Medicinal Chemistry Letters7(14):1951-1956.
Holmes et al. (1993) “Novel Dimeric Penicillin Derived Inhibitors of HIV-1 Proteinase: Interaction with the Catalytic Aspartates”Bioorganic&Medicinal Chemistry Letters3(4):503-508.
Rao et al. (1997) “Tight Binding of a Dimeric Derivative of Vancomycin with Dimeric L-Lys-D-Ala-D-Ala”J. Am. Chem. Soc. 119:10286-10290.
Tanihara et al. (1998) “Thrombin-Sensitive Peptide Linkers for Biological Signal-Responsive Drug Release Systems”Peptides19(3):421-425.
Agalarov, S., et al., (2000) “Structure of the S15, S6, S18-rRNA Complex: Assembly of the 30S Ribosome Central Domain,”Sciencevol. 288, pp. 107-112.
Agrawal, R. et al., (1998) “Visualization of Elongation Factor G on theEscherichia coli70S Ribosome: The Mechanism of Translocation,”Proc. Natl. Acad. Sci. USAvol. 95, pp. 6134-6138.
Ban, N., et al., (2000) “The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 A Resolution,”Sciencevol. 289, No. 5481, pp. 821-1096.
Ban, N., et al., (1999) “Placement of Protein and RNA Structures into a 5 A-Resolution Map of the 50S Ribosomal Subunit,”Naturevol. 400, pp. 841-847.
Ban, N., et al., (1998) “A 9 A Resolution X-Ray Crystallographic Map of the Large Ribosomal Subunit,”Cellvol. 93, pp. 1105-1115.
Baranov, P., et al., (1998) “The Database of Ribosomal Cross Links (DRC),”Nucleic Acids Researchvol. 26, No. 1, pp. 187-189.
Brodersen, D., et al., (2000) “The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit,”Cellvol. 103, pp. 1143-1154.
Brünger, A., et al., (1998) “Crystallography&NMR System: A New Software Suite for Macromolecular Structure Determination,”Acta Cryst. vol. D54, pp. 905-921.
Brünger, A., (1997) “Patterson Correlation Searches and Refinement,”Methods in Enzymology, vol. 276, pp. 558-580.
Carter, A., et al., (2001) “Crystal Structure of an Initiation Factor Bound to the 30S Ribosomal Subunit,”Sciencevol. 291, pp. 498-501.
Carter, A., et al., (2000) “Functional Insights from the Structure of the 30S Ribosomal Subunit and It's Interactions with Antibiotics,”Naturevol. 407, pp. 340-348.
Cate, J., et al., (1999) “X-Ray Crystal Structures of 70S Ribosome Functional Complexes,”Sciencevol. 285, No. 5, pp. 2095-2104.
Clemons, W. Jr., et al., (1999) “Structure of a Bacterial 30S Ribosomal Subunit at

Affiliated with

Also associated with

Rating

Modulators of ribosomal function and identification thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modulators of ribosomal function and identification thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modulators of ribosomal function and identification thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3484279