Modulation of BCL-2 phosphorylation

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

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514449, 514471, 435 723, A61K 3816, A61K 31335, A01N 4302, A01N 4308

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active

061302015

ABSTRACT:
Methods of identifying compounds that modulate bcl-2 mediated cell death are disclosed. The methods comprise contacting a cell or cell extract with a test compound and detecting the level of bcl-2 phosphorylation compared to the level of bcl-2 phosphorylation in a similar cell or cell extract not contacted with the test compound. Methods of treating an individual susceptible to or suffering from a disease characterized by inhibition of apoptosis are disclosed. The methods comprise administering to such an individual an bcl-2 phosphorylation compound that inhibits dephosphorylation of bcl-2 and/or facilitates phosphorylation of bcl-2. Methods of treating an individual susceptible to or suffering from a diseases characterized by apoptosis are disclosed. The methods comprise administering to such an individual a bcl-2 dephosphorylation compound that inhibits phosphorylation of bcl-2 and/or facilitates dephosphorylation of bcl-2.

REFERENCES:
patent: 5015568 (1991-05-01), Tsujimoto
patent: 5149628 (1992-09-01), Croce
patent: 5198338 (1993-03-01), Croce
patent: 5202429 (1993-04-01), Tsujimoto
patent: 5242795 (1993-09-01), Croce
Tripuraneni et al. "The toxin of diarrheic shellfish poisoning, okadaic acid, increases intestinal epithelial paracellular permeability" Gastroenterology. vol. 112. No. 1. pp. 100-108, 1997.
Windust et al. "Comparative toxicity of the diarrhetic shellfish poisons, okadaic acid, okadaic acid diol-ester and dinophysistoxin-4, to the diatom thalassiosira weissflogii" Toxicon. vol. 35. No. 11, pp. 1591-1603, 1997.
Rogers. B.B. "Taxol: a promising new drug of the '90s" Oncol. Nurs. forum. vol. 20. No. 10. p. 1483-9, Nov. 1993.
Haldar et al. "Antiapoptosis potential of bcl-2 oncogene by dephosphorylation" Biochem. Cell. Bio. vol. 72, pp. 455-462, 1944.
Allsopp, T., et al., "The Proto-Oncogene bcl-2 Can Selectively Rescue Neurotrophic Factor-Dependent Neurons from Apoptosis", Cell, 1993, 73, 295-307.
Alnemri, E., et al., "Overexpressed Full-length Human BCL2 Extends the Survival of Baculovirus-infected Sf9 Insect Cells", PNAS USA, 1992, 89, 7295-7299.
Alnemri, E., et al., "Involvement of BCL-2 in Glucocorticoid-induced Apoptosis of Human Pre-B-Leukemias", Cancer Research, 1992, 52, 491-492.
Bissonnette, R., et al., "Apoptotic Cell Death Induced by c-myc is Inhibited by bcl-2", Nature, 1992, 359, 552-554.
Boe, R., et al., "The Protein Phosphatase Inhibitor Okadaic Acid Induces Morphological Changes Typical of Apoptosis in Mammalian Cells", Exp. Cell. Res., 1991, 195, 237-246.
Carson, D., et al., "Oral Antilymphocyte Activity and Induction of Apoptosis by 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine", PNAS USA 1992, 89, 2970-2974.
Chen-Levy, Z., et al., "The bcl-2 Candidate Proto-Oncogene Product is a 24-Kilodalton Integral-Membrane Protein Highly Expressed in Lymphoid Cell Lines and Lymphomas Carrying the t(14,18) Translocation", Mol. And Cell. Biol., 1989, 9(2), 701-710.
Cohen, J. And Duke, "Apoptosis and Programmed Cell Death in Immunity", Annu. Rev. Immunol., 1992, 10, 267-293.
Cohen, P., "The Structure and Regulation of Protein Phosphates", Annu. Rev. Biochem., 1989, 58, 453-508.
Fernandez-Sarabia, M. And Bischoff, "Bcl-2 Associates with the ras-related Protein R-ras p23", Nature, 1993, 366, 274-275.
Gaestel, M., et al., "Dephosphorylation of the Small Heat Shock Protein hsp25 by Calcium-Calmodulin-dependent (Type 2B) Protein Phosphatase", J. Biol. Chem., 1992, 267(30), 21607-21611.
Garcia, I., et al., "Prevention of Programmed Cell Death of Sympathetic Neurons by the bcl-2 Proto-Oncogene", Science, 1992, 258, 302-304.
Gu, Y., et al., "The t(4;11) Chromosome Translocation of Human Acute Leukemias Fuses the ALL-1 Gene, Related to Drosophila trithorax, to the AF-4 Gene", Cell, 1992, 71, 701-708.
Haldar, S., et al., "Cellular Localization of the bcl-2 Protein and Response to Glucocorticoid Stress", Cell Death and Differentiation, 1994, 1, 109-115.
Haldar, S., et al., "The bcl-2 Gene Encodes A Novel G Protein", Nature, 1989, 342, 195-198.
Haldar, S., et al., "Down-regulation of bcl-2 by p53 in Breast Cancer Cells", Cancer Research, 1994, 54, 2095-2097.
Haldar, S., et al., "Purification and Characterization of the bcl-2 Protein", Archives of Biochem. And Biophysics, 1994, 315(2), 483-488.
Hengartner, M. And Horvitz, "Activation of C. Elegans Cell Death Protein CED-9 by an Amino-acid Substitution in a Domain Conserved in Bcl-2", Nature, 1994, 369, 318-320.
Hockenbery, D., et al., "Bcl-2 Functions in an Antioxidant Pathway to Prevent Apoptosis", Cell, 1993, 75, 241-251.
Hockenbery, D., et al., "Bcl-2 is an Inner Mitochondrial Membrane Protein that Blocks Programmed Cell Death", Nature, 1990, 348, 334-336.
Krajewski, S., et al., "Investigation of the Subcellular Distribution of the bcl-2 Oncoprotein: Residence in the Nuclear Envelope, Endopasmic Reticulum, and Outer Mitochondrial Membranes", Cancer Research, 1993, 53, 4701-4704.
Kyprianou, N. And Isaacs, "Thymineless Death in Androgen-independent Prostatic Cancer Cells", Biochem. And Biophys. Res. Comm., 1989, 165(1), 73-81.
McDonnell, T., et al., "bcl-2 -Immunoglobulin Transgenic Mice Demonstrate Extended B Cell Survival and Follicular Lymphoproliferation", Cell, 1989, 57, 79-88.
Miyashita, T. And Reed, "bcl-2 Gene Transfer Increases Relative Resistance of S49.1 and WEHI7.2 Lymphoid Cells to Cell Death and DNA Fragmentation Induced by Glucocorticoids and Multiple Chemotherapeutic Drugs", Cancer Research, 1992, 52, 5407-5411.
Negrini, M., et al., "Molecular Analysis of mbcl-2: Structure and Expression of the Murine Gene Homologous to the Human Gene Involved in Follicular Lymphoma", Cell, 1987, 49, 455-463.
Oltvai, Z.N., et al., "Bcl-2 Heterodimerizes In vivo with a Conserved Homolog, Bax, That Accelerates Programmed Cell Death", Cell, 1993, 74, 609-619.
Reed, J., "Bcl-2 and the Regulation of Programmed Cell Death", The J. Of Cell Biol., 1994, 124(1,2), 1-6.
Schwartzman, R. And Cidlowski, "Apoptosis: The Biochemistry and Molecular Biology of Programmed Cell Death", Endocrine Reviews, 1993, 14(2), 133-151.
Somers, J. And DeFranco, Effects of Okadaic Acid, a Protein Phosphatase Inhibitor, on Glucocorticoid Receptor-Mediated Enhancement, Molecular Endocrinology, 1992, 6, 26-34.
Strasser, A., et al., "bcl-2 Transgene Inhibits T Cell Death and Perturbs Thymic Self-censorship", Cell, 1991, 67, 889-899.
Tsujimoto, Y., et al., "Cloning of the Chromosome Breakpoint of Neopastic B Cells with the t(14;18) Chromosome Translocation", Science, 1984, 226, 1097-1099.
Tsujimoto, Y., et al., "Involvement of the bcl-2 Gene in Human Follicular Lymphoma", Science, 1985, 228, 1440-1443.
Wyllie, A.H., "Glucocorticoid-induced Thymocyte Apoptosis is Associated with Endogenous Endonuclease Activation", Nature, 1980, 284, 555-556.
Yin, et al., "BH1 and BH2 Domains of Bcl-2 Are Required for Inhibition of Apoptosis and Heterodimerization with Bax", Nature, 1994, 369, 321-323.
Bonnefoy-Berard, et al., "Calcineurin and Kinases in the Control of BCL-2 Expression and Apoptosis in Human B Cell Lines", J. Immunol., 150, No. 8, Part 2, issued May 25, 1993, p. 186A, col. 2, Abstract No. 1056.
Haldar, et al., "Antiapoptosis Potential of bcl-2 Oncogene by Dephosphorylation", Biochem. And Cell Biol., 72, No. 11-12, issued 1994, pp. 455-461.
Haldar, et al., "Inactivation of BCL-2 by phophorylation", Proc. Natl. Acad. Sci. USA, 92, No. 10, issued May 9, 1995, pp. 4507-4511.

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