Modulating the efficiency of nucleic acid amplification reaction

Details Modulating the efficiency of nucleic acid amplification reaction Modulating the efficiency of nucleic acid amplification reaction

1996-10-07

2000-05-02

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 536 221, 536 231, 536 2532, C12P 1934, C07H 2104, C07H 2100

Patent

active

060571349

ABSTRACT:
The invention relates to modified oligonucleotide primers used to adjust the amplification efficiency of an abundant target without affecting the amplification of other targets in a DNA synthesis reaction. The invention may be used in PCR.TM. or any other primer dependent DNA transcription technology.

REFERENCES:
patent: 5348853 (1994-09-01), Wang et al.
patent: 5545540 (1996-08-01), Mian
patent: 5567583 (1996-10-01), Wang et al.
Compton, "Nucleic Acid Sequence-Based Amplification," Nature, 350:91-92, 1991.
Gaudette and Crain, "A Simple Method for Quantifying Specific mRNAs in Small Numbers of Early Mouse Embryos," Nucleic Acids Research, 19(8):1879-1884, 1991.
Guatelli et al., "Isothermal in Vitro Amplification of Nucleic Acids by a Multienzyme Reaction Modeled After Retroviral Replication," PNAS, 87:1874-1878, 1990.
Holland et al., "Detection of Specific Polymerase Chain Reaction Product by Utilizing the 5'->3' Exonuclease Activity of Thermus Aquaticus DNA Polymerase," Proc. Natl. Acad. Sci. USA, 88:7276-7280, 1991.
Horikoshi et al., "Qunatitation of Thymidylate Synthesis, Dihydrofolate Reductase, and DT-Diaphoreses Gene Expression in Human Tumors Using the Polymerase Chain Reaction," Cancer Research, 52:108-116, 1992.
Lee et al., "Allelic Discrimination by Nick-Translation PCR.TM. with Fluorogenic Probes," Nucl. Acids. Research, 21:3761-3766, 1993.
Nicoletti and Sassy-Prigent, "An Alternative Quantitative Polymerase Chain Reaction Method," Anal Biochem, 236:229-241, 1996.
Orum et al., "Single Base Pair Mutation Analysis by PNA Directed PCR Clamping," Nucl. Acids Research, 21(23):5331-5336, 1993.
Wong et al., "Monitoring mRNA Expression by Polymerase Chain Reaction: The `Primer-Dropping` Method," Annal Biochem., 223:251-258, 1994.

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