Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Reexamination Certificate
2006-05-16
2010-02-23
Angell, J. E (Department: 1635)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
C435S325000, C536S024500
Reexamination Certificate
active
07666676
ABSTRACT:
The hedgehog pathway in cerebellar cancer cells was modulated with siRNA specifically targeted to the shh and gli-I genes. Silencing of the two genes in a medullablastoma cell line transfected with the siRNAs caused significant reduction of mRNA specific for the targeted shh and gli-I genes and a loss of protein expression. The disclosed methods and compositions may be useful for treatment of a range of primitive neuroectodermal tumors (PNET) by shutting down or modulating the expression of gene products associated with the hedgehog pathway.
REFERENCES:
patent: 5883124 (1999-03-01), Samid
patent: 2004/0072345 (2004-04-01), Altaba et al.
Ambion, 2 pages (http://www.ambion.com/techlib/misc/siRNA—finder.html) accessed Sep. 27, 2007, available to the public 2002.
Tuschl (Molecular Interventions, 2002; vol. 2(3), pp. 158-167).
Hallahan et al. (Cancer Research, 2004, vol. 64, pp. 7794-7800).
Jarov, A et al Developmental Biology, 2003; vol. 261, pp. 520-536.
Bedford, J.S., et al. “Applications of RNA interference for studies in DNA damage processing, genome stability, mutagenesis, and cancer”,Seminars in Cancer Biology. Apr. 28, 2003; pp. 301-308, vol. 13 No. 4.
Bertrand, Jr, J.R., et al. “Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo”.Biochemical and Biophysical Research Communications. Aug. 2, 2002; pp. 1000-1004, vol. 296 No. 4.
Borkhardt A. “Blocking oncogenes in malignant cells by RNA interference—new hope for a highly specific cancer treatment?”Cancer Cell. Sep. 2002; pp. 167-168, vol. 2 No. 3.
Brummelkamp, T.R., et al. “A system for stable expression of short interfering RNAs in mammalian cells”.Science. Apr. 19, 2002; pp. 550-553, vol. 296.
Buckingham, S.D., et al. “RNA interference: from model organisms towards therapy for neural and neuromuscular disorders”.Human Molecular Genetics. Jul. 19, 2004; pp. R275-R288, vol. 13 No. 2.
Caplen, N.J., et al. “Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells”.Ann NY Academy of Sciences, Dec. 2003; pp. 56-62, vol. 1002.
Clayton, J. “RNA interference: the silent treatment”.Nature. Sep. 30, 2004; pp. 599-605, vol. 431, No. 7008.
Damm-Welk, C., et al. “Targeting oncogenic fusion genes in leukemias and lymphomas by RNA interference”,Seminars in Cancer Biology, Aug. 2003; pp. 283-292. vol. 13 No. 4.
Dave, R.S., et al. “RNA interference: on the road to an alternate therapeutic strategy!”Reviews in Medical Virology. Nov.-Dec. 2003; pp. 373-385, vol. 13 No. 6.
Davidson, B.L., et al. “Molecular medicine for the brain: silencing of disease genes with RNA interference”.Lancet Neurology. Mar. 2004; pp. 145-149, vol. 3 No. 3.
Deveraux, Q.L., et al. “Exposing oncogenic dependencies for cancer drug target discovery and validation using RNAi”.Seminars in Cancer Biology. Aug. 2003; pp. 293-300, vol. 13 No. 4.
Dudley, N.R., et al. “RNA interference: silencing in the cytoplasm and nucleus”,Current Opinion in Molecular Therapeutics. Apr. 2003; pp. 113-117, vol. 5 No. 2.
Duursma, A.M., et al. “Ras interference as cancer therapy”.Seminars in Cancer Biology. Aug. 2003; pp. 267- 273, vol. 13 No. 4.
Duxbury, M.S. et al. “Systemic siRNA-mediated gene silencing: a new approach to targeted therapy of cancer”.Annals of Surgery. Oct. 2004; pp. 667-674, vol. 240 No. 4.
Elbashir, S.M., et al. “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells”.Nature. May 24, 2001; pp. 494-498, vol. 411.
Fan, X., et al. “Notch1 and Notch2 Have Opposite Effects on Embryonal Brain Tumor Growth”.Cancer Research. Nov. 1, 2004; pp. 7787-7793, vol. 64.
Gitlin, L., et al. “Nucleic acid-based immune system: the antiviral potential of mammalian RNA silencing”.Journal of Virology. Jul. 2003; pp. 7159-7165, vol. 77 No. 13.
Gottesman, S. “Stealth regulation: biological circuits with small RNA switches”.Genes&Development. Nov. 15, 2002; pp. 2829-2842, vol. 16 No. 22.
Hamilton, A., et al. “Two classes of short interfering RNA in RNA silencing”.EMBO Journal. Sep. 2, 2002; pp. 4671-4679, vol. 21 No. 17.
Hannon, G,J., “RNA interference”.Nature. Jul. 11, 2002; pp. 244-251, vol. 418 No. 6894.
Harborth, J., et al. “Identification of essential genes in cultured mammalian cells using small interfering RNAs”.Journal of Cell Science. 2001; pp. 4557-4565, vol. 114.
Hemann, M.T., et al. “An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo”.Nat Genet. Mar. 2003; pp. 396-400, vol. 33 No. 3.
Hommel, J.D. et al. “Local gene knockdown in the brain using viral-mediated RNA interference”.Nature Medicine. Dec. 2003; pp. 1539-1544, vol. 9 No. 12.
Hutvagner, G., et al. “RNAi: Nature abhors a double-strand”.Current Opinion in Genetics&Development. 2002; pp. 225-232, vol. 12.
Jiang, Z., et al. “Lovastatin-induced up-regulation of the BH3-only protein, Bim, and cell death in glioblastoma cells”.Journal of Neurochemistry. 2004; pp. 168-178, vol. 89 No. 1.
Jones, S.W., et al. “siRNA for gene silencing: a route to drug target discovery”.Current Opinion in Pharmacology, Aug. 17, 2004; pp. 522-527. vol. 4 No. 5.
Kittler, R., et al. “RNA interference: gene silencing in the fast lane”.Seminars in Cancer Biology. Aug. 2003; pp. 259-265, vol. 13 No. 4.
Krichevsky, A.M., et al. “RNAi functions in cultured mammalian neurons”.Proceedings of the National Academy of Sciences of the United States of America. Sep. 3, 2002; pp. 11926-9, vol. 99 No. 18.
Lapteva, N., et al. “CXCR4 knockdown by small interfering RNA abrogates breast tumor growth in vivo”.Cancer Gene Therapy. 2005; pp. 84-89, vol. 12.
Lavery, K.S., et al. “Antisense and RNAi: powerful tools in drug target discovery and validation”. Curr Opin Drug Discov Devel. Jul. 2003; pp. 561-569, vol. 6 No. 4.
Lu, P.Y., et al. “siRNA-mediated antitumorigenesis for drug target validation and therapeutics”.Curr Opin Mol Ther. Jun. 2003; pp. 225-234, vol. 5 No. 3.
McCaffrey, A.P., et al. “RNA interference in adult mice”.Nature. Jul. 4, 2002; pp. 38-39, vol. 418.
McManus, M.T., etal. “Gene silencing in mammals by small interfering RNAs”.Nat Rev Genet. Oct. 2002; pp. 737-747, vol. 3 No. 10.
Meister, G., et al. “Mechanisms of gene silencing by double-stranded RNA”.Nature. Sep. 16, 2004; pp. 343-349, vol. 431 No. 7006.
Mello, C.C., et al. “Revealing the world of RNA interference”.Nature. Sep. 16, 2004; pp. 338-342, vol. 431 No. 7006.
Milhavet, O., et al. “RNA interference in biology and medicine”.Pharmacological Review. Dec. 2003; pp. 629-648, vol. 55 No. 4.
Ning, S., et al. “siRNA-mediated down-regulation of survivin inhibits bladder cancer cell growth”.International Journal of Oncology. 2004; pp. 1065-1071, vol. 25 No. 4.
Paddison, P.J., et al. “Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells”.Genes&Development. 2002; pp. 948-958, vol. 16.
Paddison, P.J., et al. “Stable suppression of gene expression by RNAi in mammalian cells”.Proceedings of the National Academy of Sciences of the United States of America, Jan. 29, 2002; pp. 1443-1448, vol. 99 No. 3.
Paddison, P.J., et al. “RNA interference: the new somatic cell genetics?”Cancer Cell. Jul. 2002; pp. 17-23, vol. 2 No. 1.
Paddison PJ, et al. “siRNAs and shRNAs: skeleton keys to the human genome”.Curr Opin Mol Ther. Jun. 2003; pp. 217-224, vol. 5 No. 3.
Pickford AS, et al. “RNA-mediated gene silencing”.Cellular and Molecular Life Sciences, May 2003; pp. 871-882, vol. 60 No. 5.
Plasterk, R.H.A. “RNA silencing: The genome's immune system”.Science.
Braccili Selena
Lawman Michael J. P.
Angell J. E
Saliwanchik, LLoyd & Saliwanchik
St. Joseph's Children's Hospital
LandOfFree
Modulating cancer cell characteristics does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Modulating cancer cell characteristics, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modulating cancer cell characteristics will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4217884