Modulated excitation fluorescence analysis

Details Modulated excitation fluorescence analysis Modulated excitation fluorescence analysis

2004-06-17

2008-11-25

Evans, F. L (Department: 2877)

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

C356S417000, C250S458100, C436S172000

Reexamination Certificate

active

07456954

ABSTRACT:
Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.

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