Modular isotope coding approach to proteomic analysis

Details Modular isotope coding approach to proteomic analysis Modular isotope coding approach to proteomic analysis

2006-09-19

2006-09-19

Ceperley, Molly E. (Department: 1641)

Chemistry: analytical and immunological testing

Nuclear magnetic resonance, electron spin resonance or other...

C530S403000, C530S412000, C530S344000, C436S086000

Reexamination Certificate

active

07109040

ABSTRACT:
The present invention provides methods for analyzing a peptide or peptides of interest in a protein sample using a combination of a relatively generic isotope tag with a decoupled selection process, allowing simplified customization of the application with a single reagent. These methods comprise providing a first and a second protein sample; labeling the first protein sample with a first Universal Peptide Isotope Tag (U-PIT) reagent and the second protein sample with a second U-PIT reagent; separating the peptide of interest from the combined first and second protein samples; and determining the relative amount of the first U-PIT reagent and the second U-PIT reagent bound to the peptide or peptides of interest. The U-PIT label of the present inventive methods has the following general formulain-line-formulae description="In-line Formulae" end="lead"?A-B-Cin-line-formulae description="In-line Formulae" end="tail"?wherein A is a nucleophilic reactive group, B is a detectable moiety that can be isotopically labeled, and C is a charge replacement group.

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