Modular assembly retroviral vectors and uses thereof

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4353201, 4351723, 435 691, 435325, C12N 1563, C12N 1564, C12N 1567, C12N 1586

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059196670

ABSTRACT:
In accordance with the present invention, novel retroviral vectors containing modified long terminal repeats (LTRS) which enable high level and ligand-modulatable expression of a desired gene product, even after prolonged periods of cellular quiescence, have been designed and constructed. Invention vectors overcome proviral transcriptional inactivation which occurs in cultured primary cells that are growth arrested due to environmental constraints such as contact inhibition and/or nutrient starvation. Invention vectors represent a class of retroviral vectors in which LTR-promoted proviral expression in infected cells may be maintained or increased, even in situations generally considered to be non-permissive for retroviral vectors.

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