Modifying nucleotide analogues

Details Modifying nucleotide analogues Modifying nucleotide analogues

1995-03-07

1997-09-23

Witz, Jean C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 21, 435 89, 435 911, 435 912, 210632, 935 77, 935 78, C12P 1934, C12P 1930, C12N 1510

Patent

active

056703465

ABSTRACT:
Fluorescently labelled dideoxynucleoside triphosphates are widely used as chain-terminators in Sanger dideoxy sequencing operations. But these unincorporated dye-terminators migrate in an electrophoresis gel and obscure the desired sequence ladder. This invention provides a method and a kit for modifying the unincorporated dye-terminators, e.g. by removal of a 5'-triphosphate group by chemical or enzymatic means e.g. by use of a phosphatase enzyme.

REFERENCES:
Short Protocols in Molecular Biology, Ausubel et al. eds., pp. 2-4 to 2-7, 1992.
Wallace, Methods in enzymology 152:41-48 (1987).
V. Rao, Analytical Biochemistry, vol. 216, No. 1, pp. 1-14 (1994).

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