Modified β-lactamases and uses thereof

Details Modified β-lactamases and uses thereof Modified β-lactamases and uses thereof

2008-01-29

2008-01-29

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S231000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

10401867

ABSTRACT:
A tool that can screen bacteria for β-lactamases against a panel of various antibiotics is desirable. A biosensor incorporating an indicator molecule into β-lactamases may achieve this purpose, but it requires that the attached indicator molecule must not impair the binding affinity of the protein to a great extent to provide a higher sensitivity. A modified β-lactamases with a residue on the Ω-loop or outside the Ω-loop but close to the active site of β-lactamase being replaced by a reactive residue is developed in this invention.

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