Modified thermostable DNA polymerase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S183000, C435S091100

Reexamination Certificate

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07422882

ABSTRACT:
An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

REFERENCES:
patent: 0 745 675 (1996-12-01), None
patent: 0 822 256 (1998-02-01), None
patent: 0 547 359 (2002-03-01), None
patent: 05-328969 (1993-12-01), None
patent: 06-007160 (1994-01-01), None
patent: 7-298879 (1995-11-01), None
patent: 07-298879 (1995-11-01), None
patent: 10-042871 (1998-02-01), None
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
L. Blanco et al.,A general structure for DNA-dependent DNA polymerases, Gene 100, 27-38 (1991).
L. Blanco et al.,Evidence favouring the hypothesis of a conserved 3′-5′ exonuclease active site in DNA-dependent DNA polymerases, Gene 112, 139-144 (1992).
H. Kong et al.,Characterization of a DNA Polymerase from the Hyperthermophile Archaea Thermococcus litoralis, The Journal of Biological Chemistry 268(3), 1965-1975 (1993).
Fujii et al.,DNA Replication Errors Produced by the Replicative Apparatus of Escherichia coli, Journal of Molecular Biology 289, 835-850 (1999).
S. Tabor et al.,Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis, The Journal of Biological Chemistry 264 (11), 6447-6458 (1989).
T. Uemori et al.,Orangization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus, Nucleic Acids Research 21(2), 259-265 (1993).
F. B. Perler et al.,Intervening sequences in an Archaea DNA polymerase gene, Proc. Natl. Acad. Sci. USA 89, 5577-5581 (1992).
R. K. Saiki et al.,Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes, Nature 324, 163-166 (1986).
W. M. Barnes ,PCR amplification of up to 35-kb DNA with high fidelity and high yield from Λ bacteriophage templates, Proc. Natl. Acad. Sic. USA 91, 2216-2220 (1994).
Preparation and Transformation of Competent E. coli, Molecular Cloning, 2nd Edition, 1.74-1.81.
M. W. Southworth et al.,Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degree N-7 and mutations affecting 3′-5′ exonuclease activitiy, Proceedings of the National Academy of Sciences of the United States 93(11), 5281-5285 (1996).
F.C. Lawyer, et al.,High-Level Expression, Purification, and Enzymatic Characterization of Full-Length Thermus Aquaticus DNA Polymerase and a Truncated Form Deficient in 5′ to 3′ Exonuclease Activity, PCR Methods & Applications, Cold Spring Harbor Laboratory Press, US 2, 275-287 (1993).
Ruepp et al.,DNA polymerase related protein, Database Genbank Online, Accession# AL445064, Oct. 4, 2000 (abstract) ).
T. Uemori et al.,The hyperthermophilic archaeon Pyrodictium ocultum has two alpha-like DNA polymerase, Journal of Bacteriology 177(8), 2164-2177 (1995).
Crystal Structure of DNA Polymerase from Hyperthermophilic ArchaeonPyrococcus kodakaraensisKOD1, Hiroshi Hashimoto et al., J. Mol. Biol. (2001) 306, 469-477.
Crystal Structure of a Pol α Family DNA Polymerase from the Hyperthermophilic Archaeon Thermococcus sp. 9•N-7, A. Chapin Rodrigueq et al., J. Mol. Biol. (2000) 299-447-462.
Crystal structure of a thermostable type B DNA polymerase fromThermococcus gorgonarius, Karl-Peter Hopfner et al., Proc. Natl. Acad. Sci. USA, vol. 96, pp. 3600-3605, Mar. 1999, Biochemistry.
Structural Mechanism for Coordination of Proofreading and Polymerase Activities in Archaeal DNA Polymerases, Toshihiro Kuroita et al., J. Mol. Biol. (2005) 351, 291-298.
Organization and nucleotide sequence of the DNA polymerase gene from the archaeonPyrococcus furiosus, Takashi Uemori et al., 1993 Oxford University Press, Nucleic Acids Research, 1993, vol. 21. No. 2, 259-265.

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