Modified thermo-resistant DNA polymerases

Details Modified thermo-resistant DNA polymerases Modified thermo-resistant DNA polymerases

1993-11-23

1995-12-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352523, 536 232, 935 10, 935 14, C12N 912

Patent

active

054749204

ABSTRACT:
Novel, modified Taq DNA polymerases and genes encoding for them are disclosed. The modified Taq DNA polymerases of the invention are the same size, have the same heat stability and synthesis rate as the native enzyme, but lack the 5'-3' exonuclease activity. As a result of this modification, the enzymes have improved processivity as compared to the native enzyme.
The enzymes of the present invention enable improved methods of conducting PCR, DNA sequencing, and DNA synthesis.

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